Title:
SUCROSE SYNTHETASE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/005779
Kind Code:
A1
Abstract:
Provided are a sucrose synthetase and the use thereof. Compared with SEQ ID NO: 11, an amino acid sequence of the sucrose synthetase contains differences in amino acid residues selected from one or more of the following residue positions: an amino acid at position 36 being V, an amino acid at position 43 being E, an amino acid at position 179 being A, an amino acid at position 395 being R, an amino acid at position 447 being P, an amino acid at position 455 being L, and an amino acid at position 654 being R; and the sucrose synthetase has an activity not lower than that of a sucrose synthetase having an amino acid sequence as shown in SEQ ID NO: 11. By means of catalyzing the synthesis of rebaudioside A, rebaudioside D or rebaudioside M by using a glucosyltransferase and the sucrose synthetase in a combined manner, a cascade reaction is achieved, thereby providing a variety of possibilities for the selection of raw materials.
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Inventors:
WU YAN (CN)
TIAN ZHENHUA (CN)
WANG SHU (CN)
ZHENG XIAOFU (CN)
XING LITONG (CN)
TIAN ZHENHUA (CN)
WANG SHU (CN)
ZHENG XIAOFU (CN)
XING LITONG (CN)
Application Number:
PCT/CN2022/106920
Publication Date:
February 02, 2023
Filing Date:
July 21, 2022
Export Citation:
Assignee:
ABIOCHEM BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N9/10; C12N15/54; C12N15/63; C12P19/00
Domestic Patent References:
| WO2015113231A1 | 2015-08-06 |
Foreign References:
| CN111344399A | 2020-06-26 | |||
| CN202110853028A | 2021-07-27 |
Other References:
DATABASE PROTEIN ANONYMOUS : "sucrose synthase [Methylocaldum szegediense] ", XP093029574, retrieved from NCBI
WANG YAO, CHEN YI-JUN; WU XU-RI: "Advances in Enzymatic Glycosylation of Rubusoside", PHARMACEUTICAL BIOTECHNOLOGY, vol. 26, no. 4, 31 December 2019 (2019-12-31), pages 337 - 342, XP093029579, ISSN: 1005-8915, DOI: 10.19526/j.cnki.1005-8915.20190413
WU RUI, ASENCIÓN DIEZ MATÍAS D., FIGUEROA CARLOS M., MACHTEY MATÍAS, IGLESIAS ALBERTO A., BALLICORA MIGUEL A., LIU DALI: "The Crystal Structure of Nitrosomonas europaea Sucrose Synthase Reveals Critical Conformational Changes and Insights into Sucrose Metabolism in Prokaryotes", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 197, no. 17, 1 September 2015 (2015-09-01), US , pages 2734 - 2746, XP093029585, ISSN: 0021-9193, DOI: 10.1128/JB.00110-15
J. SAMBROOK, MOLECULAR CLONING: A LABORATORY MANUAL
WANG YAO, CHEN YI-JUN; WU XU-RI: "Advances in Enzymatic Glycosylation of Rubusoside", PHARMACEUTICAL BIOTECHNOLOGY, vol. 26, no. 4, 31 December 2019 (2019-12-31), pages 337 - 342, XP093029579, ISSN: 1005-8915, DOI: 10.19526/j.cnki.1005-8915.20190413
WU RUI, ASENCIÓN DIEZ MATÍAS D., FIGUEROA CARLOS M., MACHTEY MATÍAS, IGLESIAS ALBERTO A., BALLICORA MIGUEL A., LIU DALI: "The Crystal Structure of Nitrosomonas europaea Sucrose Synthase Reveals Critical Conformational Changes and Insights into Sucrose Metabolism in Prokaryotes", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 197, no. 17, 1 September 2015 (2015-09-01), US , pages 2734 - 2746, XP093029585, ISSN: 0021-9193, DOI: 10.1128/JB.00110-15
J. SAMBROOK, MOLECULAR CLONING: A LABORATORY MANUAL
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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