Title:
RECOMBINANT TYPE XVII HUMANIZED COLLAGEN EXPRESSED IN YEAST AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2024/087784
Kind Code:
A1
Abstract:
The present invention belongs to the technical field of genetic engineering and synthetic biology. Provided are a recombinant type XVII humanized collagen expressed in yeast and a preparation method therefor, and particularly provided are a recombinant humanized collagen that the 15th helical region of type XVII collagen is expressed, and a recombinant engineering bacterium and a preparation method therefor. Provided is a full-length sequence of the 15th helical region of type XVII collagen, and a mutant obtained by means of the mutation of amino acid residues on the basis of the sequence. The full-length sequence of the 15th helical region of type XVII collagen is expressed in Pichia pastoris for the first time, and a complete set of fermentation and purification processes are established to improve the protein expression level and reduce the degradation. It is verified by means of experiments that the obtained protein has biological activities such as a cell adhesion activity and cell-migration-promoting activity reaching or exceeding those of natural human collagen, can achieve the purpose of real product application of the product, and can be widely used in fields such as medicine, medical devices, biomaterials, tissue engineering and cosmetics.
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Inventors:
WANG LIPING (CN)
LI JIAJIA (CN)
QIAN CHENMING (CN)
CHENG PENGFEI (CN)
LIU HUIMIN (CN)
JIANG WENWEN (CN)
WEI XUEHUA (CN)
XU YUAN (CN)
QIAN SONG (CN)
FAN XIAOJU (CN)
LI HAIHANG (CN)
LI JIAJIA (CN)
QIAN CHENMING (CN)
CHENG PENGFEI (CN)
LIU HUIMIN (CN)
JIANG WENWEN (CN)
WEI XUEHUA (CN)
XU YUAN (CN)
QIAN SONG (CN)
FAN XIAOJU (CN)
LI HAIHANG (CN)
Application Number:
PCT/CN2023/111030
Publication Date:
May 02, 2024
Filing Date:
August 03, 2023
Export Citation:
Assignee:
JIANGSU TRAUTEC MEDICAL TECH CO LTD (CN)
International Classes:
C07K14/78; A61K8/65; A61K38/39; C07K1/18; C12N15/12; C12N15/81
Domestic Patent References:
| WO2020127929A1 | 2020-06-25 |
Foreign References:
| CN116375847A | 2023-07-04 | |||
| CN113185604A | 2021-07-30 | |||
| CN110845603A | 2020-02-28 | |||
| EP3438125A1 | 2019-02-06 |
Other References:
DATABASE UniPotKB 12 October 2022 (2022-10-12), "RecName: Full=Collagen alpha-1(XVII) chain", XP093161494, Database accession no. Q9UMD9.3
AI-MEI ZHOU: " Purification and characterization of recombinant human-source collagen", FOOD AND FERMENTATION INDUSTRIES, vol. 41, no. 3, 1 January 2015 (2015-01-01), pages 46 - 52, XP093161501
JONSSON, F. ET AL.: "Mutations in collagen, type XVII, alpha 1 (COL17A1) cause epithelial recurrent erosion dystrophy (ERED)", HUMAN MUTATION, vol. 36, no. 4, 10 February 2015 (2015-02-10), pages 463 - 473, XP071976236, DOI: 10.1002/humu.22764
AI-MEI ZHOU: " Purification and characterization of recombinant human-source collagen", FOOD AND FERMENTATION INDUSTRIES, vol. 41, no. 3, 1 January 2015 (2015-01-01), pages 46 - 52, XP093161501
JONSSON, F. ET AL.: "Mutations in collagen, type XVII, alpha 1 (COL17A1) cause epithelial recurrent erosion dystrophy (ERED)", HUMAN MUTATION, vol. 36, no. 4, 10 February 2015 (2015-02-10), pages 463 - 473, XP071976236, DOI: 10.1002/humu.22764
Attorney, Agent or Firm:
NANJING IPZHI INTELLECTUAL PROPERTYAGENCY CO..LTD (CN)
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