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Title:
PRIMER HAVING STEM-LOOP STRUCTURES AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/082624
Kind Code:
A1
Abstract:
Provided are a primer having stem-loop structures and the use thereof. The primer sequentially comprises, from the 5' end to the 3' end, primer region 1, inosines, stem-loop structure 1, primer region 2, stem-loop structure 2 and primer region 3, wherein the primer region 1, the primer region 2 and the primer region 3 are hybridized with a template to be amplified; the stem-loop structure 1 and the stem-loop structure 2 are in a linear state when the temperature is higher than 70°C and are formed when the temperature is less than or equal to 70°C; the stem-loop structures are formed before the primer region 1 and the primer region 2 bind to the template; and the stem-loop structures are not hybridized with the template. The provided primer having the stem-loop structures is suitable for multiplex PCR, enhancing the binding of the primer to a target sequence while reducing the binding of the primer to a nonspecific DNA sequence.

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Inventors:
SHANG WU (CN)
WANG YOUXIANG (CN)
YANG ZHIJIE (CN)
CHEN HAO (CN)
ZHANG YILIANG (CN)
GAN SHENGCHUN (CN)
Application Number:
PCT/CN2023/094401
Publication Date:
April 25, 2024
Filing Date:
May 16, 2023
Export Citation:
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Assignee:
NANJING PREGENE BIOMEDICINE CO LTD (CN)
International Classes:
C12N15/11; C12Q1/6886
Foreign References:
CN110283913A2019-09-27
CN103710431A2014-04-09
CN110331142A2019-10-15
CN105452485A2016-03-30
CN110923300A2020-03-27
CN109055552A2018-12-21
Other References:
LI XIONG: "Design Specific and Efficient Reverse Transcription Stem-loop Primer of MicroRNA-505", PROGRESS IN MODERN BIOMEDICINE, vol. 15, no. 27, 30 September 2015 (2015-09-30), pages 5225 - 5229, XP093160109, DOI: 10.13241/j.cnki.pmb.2015.27.007
DING, X. ET AL.: "Single Enzyme-Based Stem-Loop and Linear Primers Co-Mediated Exponential Amplification of Short Gene Sequences", ANALYTICA CHIMICA ACTA, 27 July 2019 (2019-07-27), pages 193 - 199, XP085782145, DOI: 10.1016/j.aca.2019.07.055
Attorney, Agent or Firm:
CHINABLE IP (CN)
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