METHOD FOR PRODUCING DENDRITIC CELL FORMULATION INCLUDING DENDRITIC CELLS THAT STIMULATE NKT CELLS

WIPO Patent Application WO/2024/085085

A1

[Problem] To provide a method for producing a dendritic cell formulation that stimulates NKT cells, from monocytes collected from a recipient (monocytes collected by apheresis or peripheral blood containing monocytes), and that can exhibit the effect more quickly than conventional products. [Solution] This method for producing a dendritic cell formulation comprises: an adhesion step for placing collected monocytes in a culture vessel using a liquid culture medium, and leaving the culture vessel still to cause a portion of the monocytes to adhere to the inner surface of the vessel; a non-adhering cell removal step for removing non-adhering cells including monocytes other than the cells adhering to the inner surface of the culture vessel; a differentiation step for adding a predetermined factor in the culture vessel to cause the monocytes adhering to the inner surface of the culture vessel to differentiate into immature dendritic cells; a pulsing step for performing, with α-galactosylceramide, pulsing in the culture vessel in which the immature dendritic cells are present in a non-adhering state; and an NKT-stimulating dendritic cell induction step for inducing, from the immature dendritic cells, NKT-stimulating dendritic cells that stimulate NKT cells. The dendritic cell formulation is administered back into a recipient from which the blood have been collected.

TORIKAI YUSUKE (JP)
KAGATA SHIGERU (JP)
ISHIHARA YUKI (JP)
ISHII YASUYUKI (JP)
NOZAWA RISA (JP)

PCT/JP2023/037210

April 25, 2024

October 13, 2023

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DC BIOTECH LTD (JP)

A61K35/15; A61K35/14; A61P35/00; A61P37/04; A61P43/00; C12N5/02; C12N5/0784

WO2018043660A1

2018-03-08

KUDO Ichiro (JP)

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