Title:
CUTINASE MUTANT AND USE THEREOF IN EFFICIENT DEGRADATION OF PET
Document Type and Number:
WIPO Patent Application WO/2024/046489
Kind Code:
A1
Abstract:
Provided are a cutinase mutant and the use thereof in the efficient degradation of PET. The amino acid sequence of the cutinase mutant is as shown in SEQ ID NO.2, where phenylalanine at position 239 of the amino acid sequence of the wild-type cutinase as shown in SEQ ID NO.1 is replaced with alanine. The cutinase mutant constructed in the present invention is expressed in Escherichia coli. Compared with the wild-type enzyme, the activity of the mutant enzyme shows increased stability, and improved catalytic efficiency, such that the yield of the degradation product of PET plastics (polyethylene terephthalate, PET) is increased by 42.6 times. The modified cutinase mutant improves the PET degradation efficiency.
More Like This:
Inventors:
TIAN XINPENG (CN)
SHI SONGBIAO (CN)
LI QINGLIAN (CN)
YANG JIAN (CN)
LIU YU (CN)
CENG QI (CN)
YIN JIANPING (CN)
LONG LIJUAN (CN)
SHI SONGBIAO (CN)
LI QINGLIAN (CN)
YANG JIAN (CN)
LIU YU (CN)
CENG QI (CN)
YIN JIANPING (CN)
LONG LIJUAN (CN)
Application Number:
PCT/CN2023/119278
Publication Date:
March 07, 2024
Filing Date:
September 18, 2023
Export Citation:
Assignee:
SOUTH CHINA SEA INST OCEANOLOGY CAS (CN)
International Classes:
C12N9/18; B09B3/60; C12N1/21; C12N15/55; C12N15/70; B09B101/75; C12R1/19
Domestic Patent References:
| WO2022250622A1 | 2022-12-01 |
Foreign References:
| CN116064470A | 2023-05-05 | |||
| CN114317489A | 2022-04-12 | |||
| CN115011580A | 2022-09-06 | |||
| JP2007116994A | 2007-05-17 | |||
| KR20220123831A | 2022-09-13 | |||
| US20020123123A1 | 2002-09-05 |
Other References:
DATABASE Protein 25 April 2022 (2022-04-25), ANONYMOUS: "cutinase family protein [Nocardioidaceae bacterium SCSIO 66511]", XP093143511, retrieved from NCBI Database accession no. UPK75129.1
LIU YU, LIU CHEN, LIU HUAN, ZENG QI, TIAN XINPENG, LONG LIJUAN, YANG JIAN: "Catalytic Features and Thermal Adaptation Mechanisms of a Deep Sea Bacterial Cutinase-Type Poly(Ethylene Terephthalate) Hydrolase", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, FRONTIERS RESEARCH FOUNDATION, CH, vol. 10, 26 April 2022 (2022-04-26), CH , pages 865787, XP093143508, ISSN: 2296-4185, DOI: 10.3389/fbioe.2022.865787
LIU YU, LIU CHEN, LIU HUAN, ZENG QI, TIAN XINPENG, LONG LIJUAN, YANG JIAN: "Catalytic Features and Thermal Adaptation Mechanisms of a Deep Sea Bacterial Cutinase-Type Poly(Ethylene Terephthalate) Hydrolase", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, FRONTIERS RESEARCH FOUNDATION, CH, vol. 10, 26 April 2022 (2022-04-26), CH , pages 865787, XP093143508, ISSN: 2296-4185, DOI: 10.3389/fbioe.2022.865787
Attorney, Agent or Firm:
GUANGZHOU KEYUE I.P. LAW OFFICE (CN)
Download PDF: