USE OF MODIFIED ENZYME AND COMPOSITION CONTAINING THE SAME

Title:

USE OF MODIFIED ENZYME AND COMPOSITION CONTAINING THE SAME

Document Type and Number:

Japanese Patent JPH07246093

Kind Code:

A

Abstract:

PURPOSE: To obtain a modified enzyme useful fro detoxifying the toxicity due to toxic phosphylating agents such as organophosphorus insecticides or nerve gases.

CONSTITUTION: This modified enzyme is a recombinant cholinesterase mutant; wherein a phosphylated mutant thereof is more readily reconverted to nonphosphylated mutant, than the corresponding phosphylated non-mutated cholinesterase is reconverted to non-phosphylated non-mutated cholinesterase. Detoxification by this modified enzyme involves intrinsic reactivator activity effective to reactivate the mutant by breaking the phosphyl/cholinesterase mutant covalent bond. Cholinesterase mutation is done, for example, by substituting at least one amino acid on a site in the amino acid sequence of HuAChE selected form Ser203, Glu334, His447 and others (e.g. Ser203).

Inventors:

ABUIGUDOO SHIYATSUFUAAMAN
DOBU BARAKU
ARIE OODENTORITSUCHI
CHIYANOTSUKU KURONMAN
BARUCHI BUERAN

Application Number:

JP26767594A

Publication Date:

September 26, 1995

Filing Date:

October 31, 1994

Export Citation:

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Assignee:

ISRAEL STATE

International Classes:

A61K38/45; A61P39/02; A62D3/02; B09C1/10; C12N9/18; C12N15/00; C12N15/09; C12N15/55; C12S9/00; A61K38/00; C12R1/91; (IPC1-7): C12N9/18; A61K38/45; C12N15/09

Attorney, Agent or Firm:

Kyozo Yuasa (6 people outside)

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