Title:
CULTURE MEDIUM FOR PRIMARY CELLS OF ESOPHAGEAL SQUAMOUS CARCINOMA, AND CULTIVATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2021/159560
Kind Code:
A1
Abstract:
Provided are a culture medium and cultivation method for rapidly expanding primary cells of esophageal squamous carcinoma in vitro, and the use thereof in screening drugs. The culture medium comprises an initial culture medium selected from DMEM/F12, DMEM, F12, or RPMI-1640, a Rho protease inhibitor, an antibiotic, insulin, an N2 additive, insulin-like growth factor 1, a non-essential amino acid, and optionally, hydrocortisone, optionally, glutamine, and optionally, bovine pituitary extract.
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Inventors:
LIU QINGSONG (CN)
HU JIE (CN)
CHEN CHENG (CN)
WANG WENCHAO (CN)
WANG LI (CN)
HU JIE (CN)
CHEN CHENG (CN)
WANG WENCHAO (CN)
WANG LI (CN)
Application Number:
PCT/CN2020/076736
Publication Date:
August 19, 2021
Filing Date:
February 26, 2020
Export Citation:
Assignee:
PRECEDO PHARMACEUTICALS CO LTD (CN)
International Classes:
C12N5/09
Foreign References:
| CN106190980A | 2016-12-07 | |||
| JP2014233281A | 2014-12-15 |
Other References:
LIANG, YABING ET AL.: "In Vitro 3D Culture of Esophageal Squamous Cell Carcinoma", CHINESE JOURNAL OF CELL BIOLOGY, vol. 41, no. 3, 1 April 2019 (2019-04-01), pages 456 - 460, XP055835578, ISSN: 1674-1674
KATAYAMA, M ET AL.: "Primary culture of human esophageal epithelial cells", THE TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, vol. 143, no. 2, 30 June 1984 (1984-06-30), pages 129 - 140, XP055835580, ISSN: 0040-8727
FREDDIE BRAYBSC, MSC, PHDJACQUES FERLAY, ME ET AL.: "Global Cancer Statistics 2018", CA CANCER J CLIN, vol. 68, 2018, pages 394 - 424
KARIN J. PURDIECELINE POURREYRONANDREW P. SOUTH: "Isolation and Culture of Squamous Cell Carcinoma lines. Cancer Cell Culture: Methods and Protocols", METHODS IN MOLECULAR BIOLOGY, vol. 731, 2011, pages 151 - 159
XUEFENG LIUEWA KRAWCZYK ET AL.: "Conditional reprogramming and long-term expansion of normal and tumor cells from human biospecimens", NATURE PROTOCOLS, vol. 12, no. 2, 2017, pages 439 - 451, XP037547631, DOI: 10.1038/nprot.2016.174
TODD J. JENSENCHRISTOPHER FOSTER ET AL.: "Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation", JOURNAL OF VISUALIZED EXPERIMENTS, vol. 121, March 2017 (2017-03-01), pages e55243
YUTA KASAGI: "The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial Changes", CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY, vol. 5, no. 3, 2018, pages 333 - 352
XIAODUN LIHAYLEY E. FRANCIES ET AL.: "Organoid cultures recapitulate esophageal adenocarcinoma heterogeneity providing a model for clonality studies and precision therapeutics", NATURE COMMUNICATIONS, vol. 9, 2018, pages 2983 - 2995
See also references of EP 4105320A4
KATAYAMA, M ET AL.: "Primary culture of human esophageal epithelial cells", THE TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, vol. 143, no. 2, 30 June 1984 (1984-06-30), pages 129 - 140, XP055835580, ISSN: 0040-8727
FREDDIE BRAYBSC, MSC, PHDJACQUES FERLAY, ME ET AL.: "Global Cancer Statistics 2018", CA CANCER J CLIN, vol. 68, 2018, pages 394 - 424
KARIN J. PURDIECELINE POURREYRONANDREW P. SOUTH: "Isolation and Culture of Squamous Cell Carcinoma lines. Cancer Cell Culture: Methods and Protocols", METHODS IN MOLECULAR BIOLOGY, vol. 731, 2011, pages 151 - 159
XUEFENG LIUEWA KRAWCZYK ET AL.: "Conditional reprogramming and long-term expansion of normal and tumor cells from human biospecimens", NATURE PROTOCOLS, vol. 12, no. 2, 2017, pages 439 - 451, XP037547631, DOI: 10.1038/nprot.2016.174
TODD J. JENSENCHRISTOPHER FOSTER ET AL.: "Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation", JOURNAL OF VISUALIZED EXPERIMENTS, vol. 121, March 2017 (2017-03-01), pages e55243
YUTA KASAGI: "The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial Changes", CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY, vol. 5, no. 3, 2018, pages 333 - 352
XIAODUN LIHAYLEY E. FRANCIES ET AL.: "Organoid cultures recapitulate esophageal adenocarcinoma heterogeneity providing a model for clonality studies and precision therapeutics", NATURE COMMUNICATIONS, vol. 9, 2018, pages 2983 - 2995
See also references of EP 4105320A4
Attorney, Agent or Firm:
SHANGCHENG & PARTNERS (CN)
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