Title:
SURFACE TREATMENT METHOD, SEQUENCING METHOD, AND KIT
Document Type and Number:
WIPO Patent Application WO/2024/093421
Kind Code:
A1
Abstract:
Provided are a surface treatment method, a sequencing method, and a kit for nucleic acid molecule extension. The surface treatment method comprises: contacting a phosphate compound with a surface bound with an oligonucleotide, wherein the phosphate compound comprises a compound having a structural formula represented by formula (1) and/or formula (2). With regard to the problem that bases are prone to random adsorption on the surface of the sequencing chip in the sequencing process, it has been unexpectedly discovered that compounds with a specific structure can compete with virtual terminator bases in the sequencing process for adsorption on the surface of the sequencing chip, thus reducing the random adsorption of bases on the surface of the sequencing chip, reducing the resultant sequencing error rate, and improving the sequencing throughput.
Inventors:
LU LIUYAN (CN)
WANG WEN (CN)
ZHAO NANNAN (CN)
CHEN MEIRONG (CN)
SHANG HUAN (CN)
XU DING (CN)
HE XUESEN (CN)
LIU LEI (CN)
CHEN FANG (CN)
WANG WEN (CN)
ZHAO NANNAN (CN)
CHEN MEIRONG (CN)
SHANG HUAN (CN)
XU DING (CN)
HE XUESEN (CN)
LIU LEI (CN)
CHEN FANG (CN)
Application Number:
PCT/CN2023/112186
Publication Date:
May 10, 2024
Filing Date:
August 10, 2023
Export Citation:
Assignee:
GENEMIND BIOSCIENCES COMPANY LTD (CN)
International Classes:
C12Q1/6869
Attorney, Agent or Firm:
SHENZHEN CIPRUN INTELLECTUAL PROPERTY AGENCY CO., LTD. (Zhong'ai Garden No. 040 Longzhu 7th Road, Fengjing Community, Taoyuan Sub-district, Nanshan Distric, Shenzhen Guangdong 0, CN)
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