Title:
VARIANT OF RUMINOCOCCUS GNAVUS STRAIN-DERIVED 7-BETA-HSDH, IMPROVING CONVERSION RATE FROM 7-KETO-LCA TO URSODEOXYCHOLIC ACID, AND METHOD FOR PRODUCING URSODEOXYCHOLIC ACID BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2023/075295
Kind Code:
A1
Abstract:
The present invention relates to a variant of (Ruminococcus gnavus strain-derived 7 beta-hydroxysteroid (7-beta-HSDH), improving a conversion rate from 7-keto lithocholic acid (7-keto-LCA) to ursodeoxycholic acid (UDCA), and a method for producing ursodeoxycholic acid by using same. In the present invention, the conversion efficiency of 7-Keto-LCA to UDCA has been improved through enzyme modification for development for variant production and recombinant protein production, and thus the present invention is expected to be widely used to improve UDCA biosynthesis and biosynthetic efficiency.
Inventors:
MIN BYEONG GYU (KR)
JANG HYUNG WOOK (KR)
PARK JAE BUM (KR)
KIM SO YOUNG (KR)
SOHN MIN JEONG (KR)
JANG HYUNG WOOK (KR)
PARK JAE BUM (KR)
KIM SO YOUNG (KR)
SOHN MIN JEONG (KR)
Application Number:
PCT/KR2022/016088
Publication Date:
May 04, 2023
Filing Date:
October 20, 2022
Export Citation:
Assignee:
ACE BIOPHARM CO LTD (KR)
International Classes:
C12N9/04; C12N15/70; C12P33/00
Foreign References:
| CN105274070A | 2016-01-27 | |||
| KR20190018675A | 2019-02-25 | |||
| CN111944775A | 2020-11-17 | |||
| KR20170036693A | 2017-04-03 |
Other References:
SIMONE SAVINO, ERICA ELISA FERRANDI, FEDERICO FORNERIS, STEFANO ROVIDA, SERGIO RIVA, DANIELA MONTI, ANDREA MATTEVI: "Structural and biochemical insights into 7β-hydroxysteroid dehydrogenase stereoselectivity : Stereoselective Steroid Dehydrogenation", PROTEINS: STRUCTURE, FUNCTION, AND BIOINFORMATICS, JOHN WILEY & SONS, INC., US, vol. 84, no. 6, 1 June 2016 (2016-06-01), US , pages 859 - 865, XP055491010, ISSN: 0887-3585, DOI: 10.1002/prot.25036
Attorney, Agent or Firm:
THEWAVE IP LAW FIRM (KR)
Download PDF:
Previous Patent: PORTABLE PRINTER
Next Patent: DIELECTRIC WAVEGUIDE RESONATOR AND MULTI-MODE DIELECTRIC WAVEGUIDE RESONATOR
Next Patent: DIELECTRIC WAVEGUIDE RESONATOR AND MULTI-MODE DIELECTRIC WAVEGUIDE RESONATOR