TWO-STEPS ENZYME METHOD FOR PREPARING 7-AMINOCEPHALOSPORANIC ACID

Title:

TWO-STEPS ENZYME METHOD FOR PREPARING 7-AMINOCEPHALOSPORANIC ACID

Document Type and Number:

WIPO Patent Application WO/2007/016861

Kind Code:

A1

Abstract:

The present invention discloses a two-steps enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, Wherein D-amino acid oxidase used is D-amino acid oxidase mutant of purified yeast Trigonopsis Variabilis, which having a specific activity more than 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, β -lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%.

Inventors:

WANG JUN (CN)
TSANG WAIKEI (CN)
YAP HONGKIN (CN)
CHEN JUNMIN (CN)
SIU YAULUNG (CN)
TSANG SUPYIN (CN)

Application Number:

PCT/CN2006/001940

Publication Date:

February 15, 2007

Filing Date:

August 02, 2006

Export Citation:

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Assignee:

BIORIGHT WORLDWIDE CO LTD
WANG JUN (CN)
TSANG WAIKEI (CN)
YAP HONGKIN (CN)
CHEN JUNMIN (CN)
SIU YAULUNG (CN)
TSANG SUPYIN (CN)

International Classes:

C12N9/14; C12N9/06; C12N15/70; C12N15/72; C12P21/02; C12P35/06

Domestic Patent References:

WO2003076640A2

2003-09-18

Foreign References:

CN1680558A	2005-10-12
CN1680583A	2005-10-12
CN1246147A	2000-03-01

Other References: