Title:
TRANSFORMED MICROORGANISM, AND METHOD FOR PRODUCING COPOLYMERIZED POLYESTER
Document Type and Number:
WIPO Patent Application WO/2024/058182
Kind Code:
A1
Abstract:
This transformed microorganism of a microorganism belonging to the genus Cupriavidus, which has the ability to produce a copolyester of 3-hydroxybutyric acid and another hydroxyalkanoic acid, has: a gene encoding a copolyester synthase; and a foreign gene having an amino acid sequence represented by any of SEQ ID NOs: 2-6 and encoding propionyl CoA transferase or butyl CoA transferase, and/or a foreign gene having at least 90% sequence identity to an amino acid sequence represented by any of SEQ ID Nos: 2-6, and encoding a protein having propionyl CoA transferase activity or butyl CoA transferase activity.
Inventors:
FURUTATE SHO
SATO SHUNSUKE
KOH SANGHO
TAGUCHI SEIICHI
SATO SHUNSUKE
KOH SANGHO
TAGUCHI SEIICHI
Application Number:
PCT/JP2023/033246
Publication Date:
March 21, 2024
Filing Date:
September 12, 2023
Export Citation:
Assignee:
KANEKA CORP (JP)
UNIV KOBE NAT UNIV CORP (JP)
UNIV KOBE NAT UNIV CORP (JP)
International Classes:
C12N1/21; C12N15/54; C12N15/55; C12P7/625
Foreign References:
| JP2008541719A | 2008-11-27 | |||
| JP2012516695A | 2012-07-26 | |||
| JP2012152171A | 2012-08-16 |
Other References:
DATABASE UniProtKB ANONYMOUS : "Acyl CoA:acetate/3-ketoacid CoA transferase - Epulopiscium sp |", XP093148607, retrieved from UniProt
DATABASE Uniprot ANONYMOUS : "Acetate CoA-transferase YdiF.", XP093148613
DATABASE Uniprot ANONYMOUS : "Propionate CoA-transferase - Anaerotignum lactatifermentans DSM 14214 |", XP093148616
DATABASE Uniprot ANONYMOUS : "Butyryl-CoA:acetate CoA-transferase - Anaerobutyricum hallii ", XP093148621
DATABASE Uniprot ANONYMOUS : "Butyryl-CoA:acetate CoA-transferase - Roseburia hominis ", XP093148624
NICOLE LINDENKAMP, MARC SCHÜRMANN, ALEXANDER STEINBÜCHEL: "A propionate CoA-transferase of Ralstonia eutropha H16 with broad substrate specificity catalyzing the CoA thioester formation of various carboxylic acids", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 97, no. 17, 1 September 2013 (2013-09-01), Berlin/Heidelberg, pages 7699 - 7709, XP055241903, ISSN: 0175-7598, DOI: 10.1007/s00253-012-4624-9
CHARRIER CÉDRIC, DUNCAN GARY J., REID MARTIN D., RUCKLIDGE GARRY J., HENDERSON DONNA, YOUNG PAULINE, RUSSELL VALERIE J., AMINOV RU: "A novel class of CoA-transferase involved in short-chain fatty acid metabolism in butyrate-producing human colonic bacteria", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, vol. 152, no. 1, 1 January 2006 (2006-01-01), Reading , pages 179 - 185, XP093148630, ISSN: 1350-0872, DOI: 10.1099/mic.0.28412-0
SI JAE PARK, YOUNG-AH JANG, HYUK LEE, A-REUM PARK, JUNG EUN YANG, JIHOON SHIN, YOUNG HOON OH, BONG KEUN SONG, JONGGEON JEGAL, SEUN: "Metabolic engineering of Ralstonia eutropha for the biosynthesis of 2-hydroxyacid-containing polyhydroxyalkanoates", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 20, 1 November 2013 (2013-11-01), AMSTERDAM, NL, pages 20 - 28, XP055637427, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2013.08.002
DATABASE Uniprot ANONYMOUS : "Acetate CoA-transferase YdiF.", XP093148613
DATABASE Uniprot ANONYMOUS : "Propionate CoA-transferase - Anaerotignum lactatifermentans DSM 14214 |", XP093148616
DATABASE Uniprot ANONYMOUS : "Butyryl-CoA:acetate CoA-transferase - Anaerobutyricum hallii ", XP093148621
DATABASE Uniprot ANONYMOUS : "Butyryl-CoA:acetate CoA-transferase - Roseburia hominis ", XP093148624
NICOLE LINDENKAMP, MARC SCHÜRMANN, ALEXANDER STEINBÜCHEL: "A propionate CoA-transferase of Ralstonia eutropha H16 with broad substrate specificity catalyzing the CoA thioester formation of various carboxylic acids", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 97, no. 17, 1 September 2013 (2013-09-01), Berlin/Heidelberg, pages 7699 - 7709, XP055241903, ISSN: 0175-7598, DOI: 10.1007/s00253-012-4624-9
CHARRIER CÉDRIC, DUNCAN GARY J., REID MARTIN D., RUCKLIDGE GARRY J., HENDERSON DONNA, YOUNG PAULINE, RUSSELL VALERIE J., AMINOV RU: "A novel class of CoA-transferase involved in short-chain fatty acid metabolism in butyrate-producing human colonic bacteria", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, vol. 152, no. 1, 1 January 2006 (2006-01-01), Reading , pages 179 - 185, XP093148630, ISSN: 1350-0872, DOI: 10.1099/mic.0.28412-0
SI JAE PARK, YOUNG-AH JANG, HYUK LEE, A-REUM PARK, JUNG EUN YANG, JIHOON SHIN, YOUNG HOON OH, BONG KEUN SONG, JONGGEON JEGAL, SEUN: "Metabolic engineering of Ralstonia eutropha for the biosynthesis of 2-hydroxyacid-containing polyhydroxyalkanoates", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 20, 1 November 2013 (2013-11-01), AMSTERDAM, NL, pages 20 - 28, XP055637427, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2013.08.002
Attorney, Agent or Firm:
ARCO PATENT & TRADEMARK ATTORNEYS (JP)
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