Title:
TRANSAMINASE MUTANT AND PREPARATION METHOD FOR CHIRAL AMINE COMPOUND
Document Type and Number:
WIPO Patent Application WO/2023/245815
Kind Code:
A1
Abstract:
The present invention provides a transaminase mutant and a preparation method for a chiral amine compound. The transaminase mutant comprises: (a) a protein comprising an amino acid sequence represented by SEQ ID NO: 1; or (b) a protein comprising an amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 3; or (c) a protein that undergoes an amino acid mutation at at least one of the following sites of the amino acid sequence in (b): Y89, L380, N86, Y85, T91, P83, K90, S417, S424, F301, G164, T452, and the like, and has a transaminase function; and (d) a protein having homology of more than 80% to the amino acid sequence defined in any one of (a), (b) or (c) and having a transaminase function. The problem of low activity of transaminases in the prior art can be solved. The present invention is suitable for the field of enzyme catalysis.
Inventors:
HONG HAO (US)
JAMES GAGE (US)
ZHANG NA (CN)
JIAO XUECHENG (CN)
MA YULEI (CN)
MU HUIYAN (CN)
XUAN MEIJUAN (CN)
ZHANG YANQING (CN)
ZHANG RENKUAN (CN)
JAMES GAGE (US)
ZHANG NA (CN)
JIAO XUECHENG (CN)
MA YULEI (CN)
MU HUIYAN (CN)
XUAN MEIJUAN (CN)
ZHANG YANQING (CN)
ZHANG RENKUAN (CN)
Application Number:
PCT/CN2022/109140
Publication Date:
December 28, 2023
Filing Date:
July 29, 2022
Export Citation:
Assignee:
ASYMCHEM LIFE SCIENCE TIANJIN CO LTD (CN)
International Classes:
C12N9/10; C12N15/54; C12N15/70; C12P13/00; C12P17/12
Domestic Patent References:
| WO2021081713A1 | 2021-05-06 |
Foreign References:
| CN112980810A | 2021-06-18 |
Other References:
DATABASE Protein 28 July 2019 (2019-07-28), ANONYMOUS : "aspartate aminotransferase family protein [Chromobacterium violaceum] ", XP093121384, retrieved from NCBI Database accession no. WP_011135573.1
XINXING GAO, WEI PINGHE: "Advances in molecular modification of ω-transaminase", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 34, no. 7, 25 July 2018 (2018-07-25), CN , pages 1057 - 1068, XP055762590, ISSN: 1000-3061, DOI: 10.13345/j.cjb.170455
WAN-LI ZHU, HU SHENG, LV CHANG-JIANG, ZHAO WEI-RUI, WANG HONG-PENG, MEI JIA-QI, MEI LE-HE, HUANG JUN: "A Single Mutation Increases the Thermostability and Activity of Aspergillus terreus Amine Transaminase", MOLECULES, vol. 24, no. 7, 27 March 2019 (2019-03-27), pages 1194, XP055762592, DOI: 10.3390/molecules24071194
XINXING GAO, WEI PINGHE: "Advances in molecular modification of ω-transaminase", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 34, no. 7, 25 July 2018 (2018-07-25), CN , pages 1057 - 1068, XP055762590, ISSN: 1000-3061, DOI: 10.13345/j.cjb.170455
WAN-LI ZHU, HU SHENG, LV CHANG-JIANG, ZHAO WEI-RUI, WANG HONG-PENG, MEI JIA-QI, MEI LE-HE, HUANG JUN: "A Single Mutation Increases the Thermostability and Activity of Aspergillus terreus Amine Transaminase", MOLECULES, vol. 24, no. 7, 27 March 2019 (2019-03-27), pages 1194, XP055762592, DOI: 10.3390/molecules24071194
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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