Title:
TRANSAMINASE MUTANT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/050516
Kind Code:
A1
Abstract:
Provided are a transaminase mutant and an application thereof. The transaminase mutant has an amino acid mutation based on a sequence shown in SEQ ID NO: 1, the amino acid mutation being a single position mutation from among W60Y, Y168A, V379W, V379L, V379M, C418Q and C418W, or a combination thereof. The activity, stability, and tolerance to temperature, pH and organic solvents of such transaminase mutants are improved. The present invention solves the problem of poor tolerance of extreme environments by transaminase in the prior art, and is suitable for the field of enzyme engineering.
Inventors:
HONG HAO (US)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
VYASA WILLIAMS (US)
CUI YUXIA (CN)
ZHAO JIADONG (CN)
GAO YANYAN (CN)
FU HAN (CN)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
VYASA WILLIAMS (US)
CUI YUXIA (CN)
ZHAO JIADONG (CN)
GAO YANYAN (CN)
FU HAN (CN)
Application Number:
PCT/CN2021/127138
Publication Date:
April 06, 2023
Filing Date:
October 28, 2021
Export Citation:
Assignee:
ASYMCHEM LAB TIANJIN CO LTD (CN)
International Classes:
C12N15/54; C12N9/10; C12N11/14; C12P13/00; C12P17/10
Domestic Patent References:
| WO2021135886A1 | 2021-07-08 |
Foreign References:
| CN108384767A | 2018-08-10 | |||
| CN112980810A | 2021-06-18 | |||
| CN111826362A | 2020-10-27 | |||
| CN108384767A | 2018-08-10 | |||
| CN111235140A | 2020-06-05 |
Other References:
许伟等 (XU, WEI ET AL.): "定点突变技术在转氨酶改造中的应用 (The Applications of Site-directed Mutagenesis in Aminotransferase)", 盐城工学院学报 (自然科学版) (JOURNAL OF YANCHENG INSTITUTE OF TECHNOLOGY (NATURAL SCIENCE EDITION)), vol. 17, no. 4, 31 December 2004 (2004-12-31), pages 55 - 58
JIA, DONGXU ET AL.: "Redesign of (R)-Omega-Transaminase and Its Application for Synthesizing Amino Acids with Bulky Side Chain", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 193, 4 August 2021 (2021-08-04), pages 3624 - 3640, XP037595119, DOI: 10.1007/s12010-021-03616-7
DARIUS HERBERT; SYNPHANE GIBBS; ALEXYS RIDDICK; MYRA CONWAY; MING DONG: "Crystal structure of an oxidized mutant of human mitochondrial branched‐chain aminotransferase", ACTA CRYSTALLOGRAPHICA SECTION F STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, INTERNATIONAL UNION OF CRYSTALLOGRAPHY - IUCR, OXFORD, vol. 76, no. 1, 13 January 2020 (2020-01-13), Oxford , pages 14 - 19, XP072455432, ISSN: 2053-230X, DOI: 10.1107/S2053230X19016480
JIA, DONGXU ET AL.: "Redesign of (R)-Omega-Transaminase and Its Application for Synthesizing Amino Acids with Bulky Side Chain", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 193, 4 August 2021 (2021-08-04), pages 3624 - 3640, XP037595119, DOI: 10.1007/s12010-021-03616-7
DARIUS HERBERT; SYNPHANE GIBBS; ALEXYS RIDDICK; MYRA CONWAY; MING DONG: "Crystal structure of an oxidized mutant of human mitochondrial branched‐chain aminotransferase", ACTA CRYSTALLOGRAPHICA SECTION F STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, INTERNATIONAL UNION OF CRYSTALLOGRAPHY - IUCR, OXFORD, vol. 76, no. 1, 13 January 2020 (2020-01-13), Oxford , pages 14 - 19, XP072455432, ISSN: 2053-230X, DOI: 10.1107/S2053230X19016480
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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