Title:
TRANSAMINASE, IMMOBILIZED TRANSAMINASE AND USE FOR PREPARING SITAGLIPTIN
Document Type and Number:
WIPO Patent Application WO/2022/257686
Kind Code:
A1
Abstract:
Provided is the use of an immobilized transaminase in the preparation of sitagliptin and/or (R)-3-amino-1-morpholine-4-(2,4,5-trifluorophenyl)-1-butanone. The immobilized transaminase includes resin and a transaminase, which has an amino acid sequence as shown in, for example, SEQ ID NO: 5, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 49 or a variant thereof. The variant refers to R at position 241 of the amino acid sequence as shown in SEQ ID NO: 5 being mutated to A, H, N, S, L, T, C or M. Also provided are an immobilized transaminase, and a transaminase and a preparation method therefor and the use thereof.
Inventors:
JIAO QI (CN)
HUANG YAWEI (CN)
TIAN ZHENHUA (CN)
WANG SHU (CN)
CHENG ZHANBING (CN)
SUN CHUANMIN (CN)
HUANG YAWEI (CN)
TIAN ZHENHUA (CN)
WANG SHU (CN)
CHENG ZHANBING (CN)
SUN CHUANMIN (CN)
Application Number:
PCT/CN2022/092053
Publication Date:
December 15, 2022
Filing Date:
May 10, 2022
Export Citation:
Assignee:
ABIOCHEM BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N9/10; C12N15/54; C12N15/70; C12P17/14
Domestic Patent References:
| WO2021135886A1 | 2021-07-08 | |||
| WO2020025646A1 | 2020-02-06 | |||
| WO2021077425A1 | 2021-04-29 | |||
| WO2019207443A1 | 2019-10-31 |
Foreign References:
| CN105164263A | 2015-12-16 | |||
| CN103608355A | 2014-02-26 | |||
| CN105219745A | 2016-01-06 | |||
| CN108586346A | 2018-09-28 | |||
| US20200157109A1 | 2020-05-21 |
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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