Title:
THYMIC EPITHELIAL CELL PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2023/243627
Kind Code:
A1
Abstract:
The present invention provides a thymic epithelial cell production method, the method including a step for culturing thymic epithelial progenitor cells for a long period of time. The present invention also provides a transplant therapeutic agent that includes thymic epithelial cells produced by the production method according to the present invention. The present invention further provides a T cell production method, the method including a feature of causing thymic epithelial cells produced by the production method according to the present invention to contact hematopoietic stem cells or any cells in the process of differentiation from hematopoietic stem cells to T cells.
Inventors:
HAMAZAKI YOKO (JP)
PRETEMER YANN (JP)
GAO YUXIAN (JP)
PRETEMER YANN (JP)
GAO YUXIAN (JP)
Application Number:
PCT/JP2023/021888
Publication Date:
December 21, 2023
Filing Date:
June 13, 2023
Export Citation:
Assignee:
UNIV KYOTO (JP)
International Classes:
C12N5/071; C12N5/0783
Domestic Patent References:
| WO2010143529A1 | 2010-12-16 |
Foreign References:
| JP2020534004A | 2020-11-26 |
Other References:
OTSUKA RYO, WADA HARUKA, TSUJI HYUMA, SASAKI AIRI, MURATA TOMOKI, ITOH MIZUHO, BAGHDADI MUHAMMAD, SEINO KEN-ICHIRO: "Efficient generation of thymic epithelium from induced pluripotent stem cells that prolongs allograft survival", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 10, no. 1, 14 January 2020 (2020-01-14), US , XP093118464, ISSN: 2045-2322, DOI: 10.1038/s41598-019-57088-1
GRAS-PEÑA RAFAEL; DANZL NICHOLE M.; KHOSRAVI-MAHARLOOEI MOHSEN; CAMPBELL SEAN R.; RUIZ AMANDA E.; PARKS CHRISTOPHER A.; SUEN SAVAG: "Human stem cell-derived thymic epithelial cells enhance human T-cell development in a xenogeneic thymus", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 149, no. 5, 22 October 2021 (2021-10-22), AMSTERDAM, NL , pages 1755 - 1771, XP087044952, ISSN: 0091-6749, DOI: 10.1016/j.jaci.2021.09.038
RAMOS STEPHAN A.; MORTON JOHN J.; YADAV PRABHA; REED BRENDAN; ALIZADEH SHEILA I.; SHILLEH ALI H.; PERRENOUD LONI; JAGGERS JAMES; K: "Generation of functional human thymic cells from induced pluripotent stem cells", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 149, no. 2, 29 July 2021 (2021-07-29), AMSTERDAM, NL , pages 767, XP086949947, ISSN: 0091-6749, DOI: 10.1016/j.jaci.2021.07.021
RAMOS STEPHAN A., ARMITAGE LUCAS H., MORTON JOHN J., ALZOFON NATHANIEL, HANDLER DIANA, KELLY GEOFFREY, HOMANN DIRK, JIMENO ANTONIO: "Generation of functional thymic organoids from human pluripotent stem cells", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 18, no. 4, 1 April 2023 (2023-04-01), United States , pages 829 - 840, XP093118476, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2023.02.013
GRAS-PEÑA RAFAEL; DANZL NICHOLE M.; KHOSRAVI-MAHARLOOEI MOHSEN; CAMPBELL SEAN R.; RUIZ AMANDA E.; PARKS CHRISTOPHER A.; SUEN SAVAG: "Human stem cell-derived thymic epithelial cells enhance human T-cell development in a xenogeneic thymus", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 149, no. 5, 22 October 2021 (2021-10-22), AMSTERDAM, NL , pages 1755 - 1771, XP087044952, ISSN: 0091-6749, DOI: 10.1016/j.jaci.2021.09.038
RAMOS STEPHAN A.; MORTON JOHN J.; YADAV PRABHA; REED BRENDAN; ALIZADEH SHEILA I.; SHILLEH ALI H.; PERRENOUD LONI; JAGGERS JAMES; K: "Generation of functional human thymic cells from induced pluripotent stem cells", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 149, no. 2, 29 July 2021 (2021-07-29), AMSTERDAM, NL , pages 767, XP086949947, ISSN: 0091-6749, DOI: 10.1016/j.jaci.2021.07.021
RAMOS STEPHAN A., ARMITAGE LUCAS H., MORTON JOHN J., ALZOFON NATHANIEL, HANDLER DIANA, KELLY GEOFFREY, HOMANN DIRK, JIMENO ANTONIO: "Generation of functional thymic organoids from human pluripotent stem cells", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 18, no. 4, 1 April 2023 (2023-04-01), United States , pages 829 - 840, XP093118476, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2023.02.013
Attorney, Agent or Firm:
MURAOKA, Koichiro et al. (JP)
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