Title:
SPLICING SYSTEM FOR CONTROLLING VIRUS REPLICATION IN RESPONSE TO HYPOXIC ENVIRONMENT, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/082262
Kind Code:
A1
Abstract:
Provided are a splicing system for controlling virus replication in response to a hypoxic environment, and a use thereof. The system comprises, mutually separated or fused to one another, a gene encoding an hypoxia sensing unit and a gene encoding a virus replication control unit, wherein the gene encoding the hypoxia sensing unit comprises an N-terminal splicing domain element and an oxygen-sensitive protein element which are connected to one another, and the gene encoding the virus replication control unit comprises a C-terminal splicing domain element and a virus replication key factor element which are connected via a degradation subelement. The splicing system can respond to a hypoxia condition signal, thereby achieving splicing of a virus replication key factor in response to the hypoxic environment, and exhibiting characteristics of low expression of the virus replication key factor in a normal tissue environment, and enrichment in a tumor microenvironment.
Inventors:
XU JIANQING (CN)
ZHANG XIAOYAN (CN)
DING XIANGQING (CN)
LIAO QIBIN (CN)
ZHANG XIAOYAN (CN)
DING XIANGQING (CN)
LIAO QIBIN (CN)
Application Number:
PCT/CN2022/126655
Publication Date:
April 25, 2024
Filing Date:
October 21, 2022
Export Citation:
Assignee:
SHANGHAI SINOBAY BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/62; A61K35/768; A61P35/00; C07K19/00; C12N7/01; C12R1/93
Foreign References:
| EP2239325A1 | 2010-10-13 | |||
| CN114149510A | 2022-03-08 | |||
| US20050074430A1 | 2005-04-07 | |||
| US20200270639A1 | 2020-08-27 |
Other References:
李道静等 (LI, DAOJING ET AL.): "二氢叶酸还原酶与肿瘤耐药 (Dihydrofolate Reductase And Tumor Resistance)", 生命的化学 (CHEMISTRY OF LIFE), vol. 30, no. 6, 31 December 2010 (2010-12-31)
MAZZON M. ET AL.: "A Mechanism for Induction of a Hypoxic Response by Vaccinia Virus", PNAS, vol. 110, no. 30, 23 July 2013 (2013-07-23), XP055507982, DOI: 10.1073/pnas.1302140110
IGLESIAS P. ET AL.: "A Bio-Inspired Hypoxia Sensor Using HIF1a-Oxygen-Dependent Degradation Domain", SCIENTIFIC REPORTS, 8 May 2019 (2019-05-08)
MAZZON M. ET AL.: "A Mechanism for Induction of a Hypoxic Response by Vaccinia Virus", PNAS, vol. 110, no. 30, 23 July 2013 (2013-07-23), XP055507982, DOI: 10.1073/pnas.1302140110
IGLESIAS P. ET AL.: "A Bio-Inspired Hypoxia Sensor Using HIF1a-Oxygen-Dependent Degradation Domain", SCIENTIFIC REPORTS, 8 May 2019 (2019-05-08)
Attorney, Agent or Firm:
PANAWELL & PARTNERS, LLC (CN)
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