Title:
SEPARATION METHOD AND APPARATUS FOR MICROVESICLES
Document Type and Number:
WIPO Patent Application WO/2020/249127
Kind Code:
A1
Abstract:
A microfluidic control system and method for separating flexible particles such as cell vesicles or biomacromolecules such as exosomes in a sample. The system of the present invention comprises one or more ultrahigh frequency acoustic resonators. The ultrahigh frequency acoustic resonators are capable of generating in a fluid channel an acoustic wave of which the frequency is about 0.5-50 GHz and propagated towards a wall opposite the fluid channel. By adjusting the power of the generated acoustic wave and/or the speed at which a conditioning solution flows through an acoustic wave area, flexible particles in a specified range are pushed to and remain at the top part of the flow channel in the acoustic wave area, while flexible particles outside of the specified range go downstream via the acoustic wave area to be collected, thus capturing or releasing the flexible particles in a solution such as cell vesicles or biomacromolecules, particularly exosomes.
Inventors:
DUAN XUEXIN (CN)
YANG YANG (CN)
YANG YANG (CN)
Application Number:
PCT/CN2020/096131
Publication Date:
December 17, 2020
Filing Date:
June 15, 2020
Export Citation:
Assignee:
SAVELIFE BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12M1/42; B01D21/28
Domestic Patent References:
| WO2014138739A1 | 2014-09-12 |
Foreign References:
| CN109126918A | 2019-01-04 | |||
| US10155222B2 | 2018-12-18 | |||
| CN104195028A | 2014-12-10 | |||
| CN106914288A | 2017-07-04 | |||
| CN107979352A | 2018-05-01 | |||
| US20160008532A1 | 2016-01-14 |
Other References:
CUI, WEIWEI ET AL.: "Bulk Acoustic Wave Resonator Integrated Microfluidics for Rapid and High Efficience Fluids Mixing and Bioparticle Trapping", IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM, 3 November 2016 (2016-11-03), pages 1 - 3, XP032988257, ISSN: 1948-5727
CUI, WEIWEI ET AL.: "Theoretical and Experimental Characterizations of Gigahertz Acoustic Streaming in Microscale Fluids", NANOTECHNOLOGY AND PRECISION ENGINEERING, vol. 2, 31 March 2019 (2019-03-31), pages 15 - 22, XP055764613
WU, MENGXI ET AL.: "Isolation of Exosomes from Whole Blood by Integrating Acoustics and Microfluidics", PNAS, vol. 114, no. 40, 3 October 2017 (2017-10-03), XP055713992
CUI, WEIWEI ET AL.: "Theoretical and Experimental Characterizations of Gigahertz Acoustic Streaming in Microscale Fluids", NANOTECHNOLOGY AND PRECISION ENGINEERING, vol. 2, 31 March 2019 (2019-03-31), pages 15 - 22, XP055764613
WU, MENGXI ET AL.: "Isolation of Exosomes from Whole Blood by Integrating Acoustics and Microfluidics", PNAS, vol. 114, no. 40, 3 October 2017 (2017-10-03), XP055713992
Attorney, Agent or Firm:
BEIJING QIKUN INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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