Title:
RECOMBINANT YEAST STRAIN HAVING STEROL PRODUCTIVITY, PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/022847
Kind Code:
A1
Abstract:
The present invention relates to a recombinant yeast strain having sterol productivity, a preparation method therefor and a use thereof and, more specifically, to: a recombinant yeast strain capable of producing cholesterol and cholesterol precursors in a high yield through the deletion of ERG5 and ERG6 genes and the introduction of DHCR24 and DHCR7 genes by codon-optimizing same in multiple or with a codon context method; and a production method therefor and a use thereof. In addition, disclosed are: a method for producing a recombinant yeast strain with increased production yields of cholesterol and cholesterol precursors by the additional introduction of gene tHMG1, ERG2, ERG3, ERG27, or UPC2-1 in the prepared recombinant yeast strain; and a use thereof.
Inventors:
KANG HYUN AH (KR)
MOON HYE YUN (KR)
JANG HUI JEONG (KR)
JUNG SEON A (KR)
MOON HYE YUN (KR)
JANG HUI JEONG (KR)
JUNG SEON A (KR)
Application Number:
PCT/KR2019/009360
Publication Date:
January 30, 2020
Filing Date:
July 26, 2019
Export Citation:
Assignee:
DAE WOONG PHARMA (KR)
UNIV CHUNG ANG IND ACAD COOP FOUND (KR)
UNIV CHUNG ANG IND ACAD COOP FOUND (KR)
International Classes:
C12N15/81; C12N9/02; C12N9/10; C12N15/69; C12P33/06
Foreign References:
| KR20160024353A | 2016-03-04 | |||
| CN106754993A | 2017-05-31 | |||
| KR20180087372A | 2018-08-01 | |||
| KR100418187B1 | 2004-02-11 |
Other References:
SHIVAPURKAR, R.: "An efficient method for the production of isotopically enriched cholesterol for NMR", JOURNAL OF LIPID RESEARCH, vol. 52, no. 5, 26 February 2011 (2011-02-26), pages 1062 - 1065, XP055679602
GUO, X.-J.: "Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction", BIOTECHNOLOGY FOR BIOFUELS, vol. 11, no. 1, 16 June 2018 (2018-06-16), pages 1 - 14, XP055615428
DATABASE Nucleotide 21 September 2015 (2015-09-21), "PREDICTED: Pseudopodoces humilis 7-dehydrocholesterol reductase (DHCR7), transcript variant X4, mRNA", XP055679606, retrieved from NCBI Database accession no. XM 014260434.1
S OUZA, C. M.: "A stable yeast strain efficiently producing cholesterol instead of ergosterol is functional for tryptophan uptake, but not weak organic acid resistance", METABOLIC ENGINEERING, vol. 13, 30 June 2011 (2011-06-30), pages 555 - 569, XP028274823, DOI: 10.1016/j.ymben.2011.06.006
See also references of EP 3848463A4
GUO, X.-J.: "Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction", BIOTECHNOLOGY FOR BIOFUELS, vol. 11, no. 1, 16 June 2018 (2018-06-16), pages 1 - 14, XP055615428
DATABASE Nucleotide 21 September 2015 (2015-09-21), "PREDICTED: Pseudopodoces humilis 7-dehydrocholesterol reductase (DHCR7), transcript variant X4, mRNA", XP055679606, retrieved from NCBI Database accession no. XM 014260434.1
S OUZA, C. M.: "A stable yeast strain efficiently producing cholesterol instead of ergosterol is functional for tryptophan uptake, but not weak organic acid resistance", METABOLIC ENGINEERING, vol. 13, 30 June 2011 (2011-06-30), pages 555 - 569, XP028274823, DOI: 10.1016/j.ymben.2011.06.006
See also references of EP 3848463A4
Attorney, Agent or Firm:
ERUUM & LEEON INTELLECTUAL PROPERTY LAW FIRM (KR)
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