Title:
PRODUCTION OF VIRUS VECTOR PLASMID IN BACILLUS SUBTILIS
Document Type and Number:
WIPO Patent Application WO/2022/097646
Kind Code:
A1
Abstract:
The present disclosure pertains to production of a virus vector plasmid in Bacillus subtilis. According to one aspect, the present disclosure provides a method for producing a virus vector plasmid having a sequence to be replicated in Bacillus subtilis. The method includes a step for forming a plasmid in a host cell by introducing, into the host cell, a nucleic acid that has a sequence to be replicated in Bacillus subtilis and that includes a nucleic acid sequence for producing a virus vector. In one embodiment, Bacillus subtilis could have the ability to form a plasmid from a nucleic acid acquired from outside, and therefore, in this method, the nucleic acid introduced dose not have be a plasmid.
Inventors:
SAITO SHUNSUKE (JP)
TSUGE KENJI (JP)
TSUGE KENJI (JP)
Application Number:
PCT/JP2021/040410
Publication Date:
May 12, 2022
Filing Date:
November 02, 2021
Export Citation:
Assignee:
SYNPLOGEN CO LTD (JP)
International Classes:
C12N15/70; C12N15/75; C12N15/81
Domestic Patent References:
| WO2001090392A1 | 2001-11-29 | |||
| WO2015111248A1 | 2015-07-30 |
Foreign References:
| JP2002528049A | 2002-09-03 | |||
| US20180155740A1 | 2018-06-07 | |||
| US5908845A | 1999-06-01 | |||
| JP4479199B2 | 2010-06-09 | |||
| JP2018093024A | 2018-06-14 | |||
| JP2020184495A | 2020-11-12 |
Other References:
TSUGE, K. ET AL.: "One step assembly of multiple DNA fragments with a designed order and orientation in Bacillus subtilis plasmid", NUCLEIC ACIDS RESEARCH, vol. 31, no. 21, 2003, pages e133, XP002569369, DOI: 10.1093/NAR/GNG133
ITAYA, M. ET AL.: "Construction and Manipulation of Giant DNA by a Genome Vector", METHODS IN ENZYMOLOGY, vol. 498, 2011, pages 427 - 447, XP055355825, DOI: 10.1016/B978-0-12-385120-8.00019-X
JANNIERE, L.A. GRUSSS. D. EHRLICH: "Plasmids", 1993, AMERICAN SOCIETY FOR MICROBIOLOGY, article "Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology and molecular genetics", pages: 625 - 644
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1995, WILEY INTERSCIENCE PUBLISHERS, article "Molecular Cloning"
TSUGE, K.ITAYA, M.: "Recombinational transfer of 100-kilobase genomic DNA to plasmid in Bacillus subtilis 168", JOURNAL OF BACTERIOLOGY, vol. 183, 2001, pages 5453 - 5458, XP002545781, DOI: 10.1128/JB.183.18.5453-5458.2001
WILD JHRADECNA ZSZYBALSKI W: "Conditionally Amplifiable BACs: Switching From Single-Copy to High-Copy Vectors and Genomic Clones", GENOME RES, vol. 12, 2002, pages 1434 - 1444, XP002463965, DOI: 10.1101/gr.130502
ANAGNOSTOPOULOU, C.SPIZIZEN, J., J. BACTERIOL., vol. 81, 1961, pages 741 - 746
IMANAKA, T. ET AL., J. GEN. MICROBIOI., vol. 130, 1984, pages 1399 - 1408
TANAKA, T.M. OGURA: "A novel Bacillus natto plasmid pLS32 capable of replication in Bacillus subtilis", FEBS LETT, vol. 422, 1998, pages 243 - 246, XP071237222, DOI: 10.1016/S0014-5793(98)00015-5
SWINFIELD, T. J. ET AL., GENE, vol. 87, 1990, pages 79 - 90
YANSURA, D.HENNER, D.J., PRO. NATL. ACAD. SCI, USA, vol. 81, 1984, pages 439 - 443
ITAYA, M, BIOSCI. BIOTECHNOL. BIOCHEM., vol. 63, 1999, pages 602 - 604
IMANAKA, T. ET AL., J.GEN.MICROBIOI., vol. 130, 1984, pages 1399 - 1408
TANAKA, TOGRA, M., FEBS LETT., vol. 422, 1998, pages 243 - 246
TSUGE, K.SATO, Y.KOBAYASHI, Y.GONDO, M.HASEBE, M.TOGASHI, T.TOMITA, M.ITAYA, M.: "Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments", SCIENTIFIC REPORTS, vol. 5, 2015, pages 10655, XP055369781, DOI: 10.1038/srep10655
SAMBROOK, J. ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
ITAYA, M. ET AL.: "Construction and Manipulation of Giant DNA by a Genome Vector", METHODS IN ENZYMOLOGY, vol. 498, 2011, pages 427 - 447, XP055355825, DOI: 10.1016/B978-0-12-385120-8.00019-X
JANNIERE, L.A. GRUSSS. D. EHRLICH: "Plasmids", 1993, AMERICAN SOCIETY FOR MICROBIOLOGY, article "Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology and molecular genetics", pages: 625 - 644
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1995, WILEY INTERSCIENCE PUBLISHERS, article "Molecular Cloning"
TSUGE, K.ITAYA, M.: "Recombinational transfer of 100-kilobase genomic DNA to plasmid in Bacillus subtilis 168", JOURNAL OF BACTERIOLOGY, vol. 183, 2001, pages 5453 - 5458, XP002545781, DOI: 10.1128/JB.183.18.5453-5458.2001
WILD JHRADECNA ZSZYBALSKI W: "Conditionally Amplifiable BACs: Switching From Single-Copy to High-Copy Vectors and Genomic Clones", GENOME RES, vol. 12, 2002, pages 1434 - 1444, XP002463965, DOI: 10.1101/gr.130502
ANAGNOSTOPOULOU, C.SPIZIZEN, J., J. BACTERIOL., vol. 81, 1961, pages 741 - 746
IMANAKA, T. ET AL., J. GEN. MICROBIOI., vol. 130, 1984, pages 1399 - 1408
TANAKA, T.M. OGURA: "A novel Bacillus natto plasmid pLS32 capable of replication in Bacillus subtilis", FEBS LETT, vol. 422, 1998, pages 243 - 246, XP071237222, DOI: 10.1016/S0014-5793(98)00015-5
SWINFIELD, T. J. ET AL., GENE, vol. 87, 1990, pages 79 - 90
YANSURA, D.HENNER, D.J., PRO. NATL. ACAD. SCI, USA, vol. 81, 1984, pages 439 - 443
ITAYA, M, BIOSCI. BIOTECHNOL. BIOCHEM., vol. 63, 1999, pages 602 - 604
IMANAKA, T. ET AL., J.GEN.MICROBIOI., vol. 130, 1984, pages 1399 - 1408
TANAKA, TOGRA, M., FEBS LETT., vol. 422, 1998, pages 243 - 246
TSUGE, K.SATO, Y.KOBAYASHI, Y.GONDO, M.HASEBE, M.TOGASHI, T.TOMITA, M.ITAYA, M.: "Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments", SCIENTIFIC REPORTS, vol. 5, 2015, pages 10655, XP055369781, DOI: 10.1038/srep10655
SAMBROOK, J. ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
YAMAMOTO, Shusaku et al. (JP)
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