Title:
PRODUCTION METHOD FOR FOREIGN PROTEIN USING ESCHERICHIA COLI
Document Type and Number:
WIPO Patent Application WO/2022/201917
Kind Code:
A1
Abstract:
The present invention makes it possible to efficiently extract and purify a target foreign protein expressed using Escherichia coli from the periplasm. A protein production method that includes steps (a)–(c). (a) A step for introducing a gene that codes for a target foreign protein into Escherichia coli in which a gene associated with maintenance of outer membrane structure has been modified. (b) A step for culturing the Escherichia coli from (a). (c) A step for collecting the target foreign protein from the periplasmic fraction of the cultured bacterial cells.
Inventors:
YAMAGIWA RAIKA (JP)
TOKUNAGA TOMOHISA (JP)
NISHIYAMA TOZO (JP)
MINAKUCHI KAZUNOBU (JP)
TOKUNAGA TOMOHISA (JP)
NISHIYAMA TOZO (JP)
MINAKUCHI KAZUNOBU (JP)
Application Number:
PCT/JP2022/004680
Publication Date:
September 29, 2022
Filing Date:
February 07, 2022
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C07K1/14; C12N1/21; C12N15/31; C12N15/70; C12P21/00
Domestic Patent References:
| WO2013016428A2 | 2013-01-31 | |||
| WO1998014600A1 | 1998-04-09 |
Foreign References:
| JPH08242879A | 1996-09-24 | |||
| JPH08242879A | 1996-09-24 | |||
| JP2021046808A | 2021-03-25 |
Other References:
"Advances in Applied Biotechnology", 20 January 2012, INTECH, ISBN: 978-953-30-7820-5, article WAEGEMAN HENDRIK, DE MARJAN: "Increasing Recombinant Protein Production in E. coli by an Alternative Method to Reduce Acetate", XP055971207, DOI: 10.5772/30726
YANG HAIQUAN; WANG FUXIANG; WANG HAOKUN; LU XIAO; SHEN WEI; CHEN XIANZHONG: "DeletingmrdAandmrcBto significantly improve extracellular recombinant protein production inEscherichia coli", BIOCHEMICAL ENGINEERING JOURNAL, ELSEVIER, AMSTERDAM, NL, vol. 143, 1 January 1900 (1900-01-01), NL , pages 185 - 195, XP085604541, ISSN: 1369-703X, DOI: 10.1016/j.bej.2019.01.003
ZHAO-YUAN CHEN, JIE CAO, LI XIE, XIAO-FEI LI, ZHEN-HAI YU, WANG-YU TONG: "Construction of leaky strains and extracellular production of exogenous proteins in recombinant E scherichia coli : Extracellular production of exogenous proteins", MICROBIAL BIOTECHNOLOGY, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 7, no. 4, 1 July 2014 (2014-07-01), GB , pages 360 - 370, XP055561732, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12127
C. SCHIMEK ET AL., BIOTECHNOL. PROGRESS, vol. 36, 2020, pages e2999
FEMS MICROBIOLOGY LETTERS, vol. 165, 1998, pages 335 - 340
OURNAL OF BACTERIOLOGY, December 1995 (1995-12-01), pages 7171 - 7177
CURR. GENET., vol. 10, no. 8, 1986, pages 573 - 578
DATSENKO K.AWANNER B.L, PROC. NATL. ACAD. SCI., U.S.A, vol. 97, no. 12, 2000, pages 6640 - 6645
LINK A. J ET AL., J. BACTERIOL., vol. 179, 1997, pages 6228 - 6237
CASCALES ELLOUBES R., MOL. MICROBIOL., vol. 51, no. 3, 2004, pages 873 - 885
YANG HAIQUAN; WANG FUXIANG; WANG HAOKUN; LU XIAO; SHEN WEI; CHEN XIANZHONG: "DeletingmrdAandmrcBto significantly improve extracellular recombinant protein production inEscherichia coli", BIOCHEMICAL ENGINEERING JOURNAL, ELSEVIER, AMSTERDAM, NL, vol. 143, 1 January 1900 (1900-01-01), NL , pages 185 - 195, XP085604541, ISSN: 1369-703X, DOI: 10.1016/j.bej.2019.01.003
ZHAO-YUAN CHEN, JIE CAO, LI XIE, XIAO-FEI LI, ZHEN-HAI YU, WANG-YU TONG: "Construction of leaky strains and extracellular production of exogenous proteins in recombinant E scherichia coli : Extracellular production of exogenous proteins", MICROBIAL BIOTECHNOLOGY, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 7, no. 4, 1 July 2014 (2014-07-01), GB , pages 360 - 370, XP055561732, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12127
C. SCHIMEK ET AL., BIOTECHNOL. PROGRESS, vol. 36, 2020, pages e2999
FEMS MICROBIOLOGY LETTERS, vol. 165, 1998, pages 335 - 340
OURNAL OF BACTERIOLOGY, December 1995 (1995-12-01), pages 7171 - 7177
CURR. GENET., vol. 10, no. 8, 1986, pages 573 - 578
DATSENKO K.AWANNER B.L, PROC. NATL. ACAD. SCI., U.S.A, vol. 97, no. 12, 2000, pages 6640 - 6645
LINK A. J ET AL., J. BACTERIOL., vol. 179, 1997, pages 6228 - 6237
CASCALES ELLOUBES R., MOL. MICROBIOL., vol. 51, no. 3, 2004, pages 873 - 885
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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