PRIMER-MOLECULAR BEACON COMBINATION FOR MOLECULAR TYPING OF WILD TYPE AND MUTANT TYPE OF MONKEY POXVIRUS AND USE THEREOF

WIPO Patent Application WO/2024/055627

A1

The present application provides a primer-molecular beacon combination for molecular typing of a wild type and a mutant type of a monkey poxvirus and use thereof. The primer-molecular beacon combination comprises a primer pair and a molecular beacon that specifically amplify and detect a monkey poxvirus F3L gene, respectively. Nucleotide sequences of the primer pair that specifically amplifies the monkey poxvirus F3L gene are set forth in SEQ ID No. 1 and SEQ ID No. 2. The primer-molecular beacon combination of the present application features good specificity and high detection sensitivity, and the detection lower limit reaches 1 copy. Rapid, simple, convenient, accurate, efficient, practical, and economical detection of a wild type of monkey poxvirus and variants thereof can be realized by using the kit of the present application, and the wild type of the monkey poxvirus and variants thereof can also be effectively distinguished by means of a molecular beacon melting curve method, which can meet the requirements of related pathogen detection in actual clinical examination.

YANG WENWEN (CN)
ZHOU KAIYUE (CN)
TAO WEIWEI (CN)
DOU JINGMENG (CN)
HE XIAOMING (CN)
LIN LING (CN)

PCT/CN2023/095663

March 21, 2024

May 23, 2023

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SHANGHAI BIOTECAN PHARMACEUTICALS CO LTD (CN)
SHANGHAI HUATENG BIOTECHNOLOGY CO LTD (CN)
SHANGHAI BAOTENG MEDICAL LABORATORY CO LTD (CN)

C12Q1/68; C12N15/11; C12R1/93

CN116254371A	2023-06-13
CN101407850A	2009-04-15
CN103468830A	2013-12-25
CN106521032A	2017-03-22
CN102839224A	2012-12-26
CN112779354A	2021-05-11
CN114752711A	2022-07-15

BEYOND ATTORNEYS AT LAW (CN)

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