Title:
PENICILLIN G ACYLASE MUTANT HAVING INCREASED CEFAZOLINE PRODUCTIVITY AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/096298
Kind Code:
A1
Abstract:
The present invention relates to a penicillin G acylase mutant having increased cefazoline productivity and a use thereof, wherein Achromobacter sp. CCM 4824-derived penicillin G acylase mutants having unique mutation forms disclosed in the present invention have a very high synthesis activity specifically for cefazoline, compared to wild-type enzyme thereof and other known mutant forms, and have a remarkably high synthesis ratio (S/H ratio) compared to the degradation activity of a substrate used for synthesis of cefazoline, and thus the productivity of cefazoline is remarkably increased to a significant level in industrial applications.
Inventors:
SHIN YONG CHUL (KR)
PIAO ZHE (KR)
WANG EUN SEON (KR)
YUN YOUNG SUNG (KR)
KIM NA RI (KR)
KANG MI SUK (KR)
PIAO ZHE (KR)
WANG EUN SEON (KR)
YUN YOUNG SUNG (KR)
KIM NA RI (KR)
KANG MI SUK (KR)
Application Number:
PCT/KR2020/016010
Publication Date:
May 20, 2021
Filing Date:
November 13, 2020
Export Citation:
Assignee:
AMICOGEN INC (KR)
International Classes:
C12N9/84; C12P35/04
Domestic Patent References:
| WO2017143944A1 | 2017-08-31 |
Foreign References:
| KR101985911B1 | 2019-06-04 | |||
| KR20000053041A | 2000-08-25 | |||
| KR20110099137A | 2011-09-06 | |||
| KR20180004169A | 2018-01-10 |
Other References:
DATABASE Protein 1 September 2005 (2005-09-01), ANONYMOUS: "penicillin G acylase [Achromobacter sp. CCM 4824]", XP055811695, retrieved from NCBI Database accession no. AAY25991
Attorney, Agent or Firm:
LEE, Hee Sook (KR)
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