Title:
PENICILLIN EXPANDASE MUTANT, DNA ENCODING MUTANT, AND REAGENT KIT CONTAINING MUTANT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/181809
Kind Code:
A1
Abstract:
Provided is a penicillin expandase mutant, wherein same takes SEQ ID NO: 2 of the sequence listing as the reference sequence, and has the mutations of at least one of threonine at position 42 and glutamine at position 126. Also provided are the DNA encoding the mutant, and the reagent kit containing the mutant and the use thereof. The penicillin expandase mutant has an improved catalytic activity and heat resisting property.
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Inventors:
LUI YUK SUN (CN)
CHENG SHIU MING (CN)
SIU YAU LUNG (CN)
WANG JUN (CN)
CHENG SHIU MING (CN)
SIU YAU LUNG (CN)
WANG JUN (CN)
Application Number:
PCT/CN2017/077858
Publication Date:
October 26, 2017
Filing Date:
March 23, 2017
Export Citation:
Assignee:
BIORIGHT WORLDWIDE CO LTD (GB)
LUI YUK SUN (CN)
LUI YUK SUN (CN)
International Classes:
C12N9/02; C12N15/53; C12P35/00
Domestic Patent References:
| WO1998002551A2 | 1998-01-22 | |||
| WO2007023369A1 | 2007-03-01 |
Foreign References:
| CN1965083A | 2007-05-16 | |||
| CN1446908A | 2003-10-08 | |||
| CN1448506A | 2003-10-15 | |||
| CN101040043A | 2007-09-19 |
Other References:
DATABASE GENBANK 11 November 2015 (2015-11-11), KOVACEVIC, S. ET AL., XP055582027, retrieved from PROTEIN Database accession no. P18548
JI, JUNJIE: "Research Progress of Directed modification of Streptomyces Clavuligerus Penicillin Expandase", JIANGSU AGRICULTURAL SCIENCES, vol. 41, no. 7, 31 July 2013 (2013-07-31), pages 13 - 15, XP055580863, ISSN: 1002-1302
See also references of EP 3453756A4
JI, JUNJIE: "Research Progress of Directed modification of Streptomyces Clavuligerus Penicillin Expandase", JIANGSU AGRICULTURAL SCIENCES, vol. 41, no. 7, 31 July 2013 (2013-07-31), pages 13 - 15, XP055580863, ISSN: 1002-1302
See also references of EP 3453756A4
Attorney, Agent or Firm:
TEE&HOWE INTELLECTUAL PROPERTY ATTORNEYS (CN)
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