Title:
OLEAGINOUS YEAST YARROWIA LIPOLYTICA SUR2 MUTANT STRAIN AND METHOD FOR PRODUCING AND SECRETING, ONTO CELL SURFACES, SPHINGOID-BASED PRECURSORS, SPHINGOSINE AND SPHINGOLIPIDS BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2023/003350
Kind Code:
A1
Abstract:
The present invention relates to an oleaginous yeast Yarrowia lipolytica sur2 mutant strain and a method for producing and secreting, onto cell surfaces, sphingoid-based precursors, sphingosine and sphingolipids by using same. It has been identified that when a ceramidase gene is additionally introduced into a Ylsur2 deficient mutant strain, the secretion and production of dihydrosphingosine and sphingosine are increased and, further, it is has been identified that when a Ylsld1 sur2 double mutant strain in which a SLD1 gene is additionally deficient in a Ylsur2 deficient mutant strain is prepared, growth recovery and sphingosine and human glucosylceramide secretion and production are increased. It has been identified through the present invention that large amounts of dihydrosphingosine and glucosylceramide are secreted onto cell surfaces in a mutant strain in which a SUR2 gene is inactivated in Yarrowia lipolytica, which is a yeast having proven stability and excellent productivity of lipid components, and thus the possibility of mass production of a sphingosine precursor dihydrosphingosine and various forms of sphingoid-based ceramides is disclosed.
More Like This:
Inventors:
KANG HYUN AH (KR)
MOON HYE YUN (KR)
PARK HAEEUN (KR)
SHIN SEO HYEON (KR)
MOON HYE YUN (KR)
PARK HAEEUN (KR)
SHIN SEO HYEON (KR)
Application Number:
PCT/KR2022/010607
Publication Date:
January 26, 2023
Filing Date:
July 20, 2022
Export Citation:
Assignee:
UNIV CHUNG ANG IND ACAD COOP FOUND (KR)
International Classes:
C12N1/16; C12N9/80; C12N15/81; C12P13/00; C12P17/06
Domestic Patent References:
| WO2020054934A1 | 2020-03-19 | |||
| WO2007131720A1 | 2007-11-22 |
Foreign References:
| KR20080073780A | 2008-08-11 |
Other References:
DATABASE NUCLEOTIDE ANONYMOUS : "Yarrowia lipolytica CLIB122 chromosome E complete sequence", XP093026516, retrieved from NCBI
HAN CHANGPYO, JANG MINJEONG, KIM MIN JU, HAN MAN‐HO, LEE KYEONG‐RYOON, HAHN JI‐SOOK, AHN JUNGOH: "Engineering Yarrowia lipolytica for de novo production of tetraacetyl phytosphingosine", JOURNAL OF APPLIED MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 130, no. 6, 1 June 2021 (2021-06-01), GB , pages 1981 - 1992, XP093026513, ISSN: 1364-5072, DOI: 10.1111/jam.14931
HAN CHANGPYO, JANG MINJEONG, KIM MIN JU, HAN MAN‐HO, LEE KYEONG‐RYOON, HAHN JI‐SOOK, AHN JUNGOH: "Engineering Yarrowia lipolytica for de novo production of tetraacetyl phytosphingosine", JOURNAL OF APPLIED MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 130, no. 6, 1 June 2021 (2021-06-01), GB , pages 1981 - 1992, XP093026513, ISSN: 1364-5072, DOI: 10.1111/jam.14931
Attorney, Agent or Firm:
TAEBAEK INTELLECTUAL PROPERTY LAW FIRM (KR)
Download PDF: