Title:
NOVEL PRODUCTION METHOD FOR NEURAL STEM CELL/NEURAL PROGENITOR CELL
Document Type and Number:
WIPO Patent Application WO/2024/085215
Kind Code:
A1
Abstract:
Although differentiating pluripotent stem cells by temporarily differentiating the pluripotent stem cells into CTraS considerably increases the subsequent cell differentiation efficiency, it is difficult to stably induce pluripotent stem cells into CTraS in a reproducible manner, and thus, there is a problem in that, consequently, the efficiency of inducing the pluripotent stem cells to differentiate into target neural cells is low. In addition, in the case in which pluripotent stem cells need to be differentiated into target neural cells in the future, it is necessary to differentiate the pluripotent stem cells into the neural cells in a more efficient manner. The inventors of the present invention found a novel method capable of inducing pluripotent stem cells into a state in which the pluripotent stem cells are stable and easily induced to differentiate into neural cells in the future, and thus, demonstrated that the above problem can be solved. Specifically, the inventors demonstrated that the above problem can be solved by culturing cells for five days by adding four types of small molecules, namely, SB431542, LDN193189, SU5402, and EC23.
Inventors:
LIN YU-CHING ZACHARY (JP)
TANOKASHIRA DAISUKE (JP)
TANOKASHIRA DAISUKE (JP)
Application Number:
PCT/JP2023/037824
Publication Date:
April 25, 2024
Filing Date:
October 19, 2023
Export Citation:
Assignee:
K PHARMA INC (JP)
International Classes:
C12N5/0735; C12N5/0793; C12N5/0797; C12N5/10
Domestic Patent References:
| WO2022051847A1 | 2022-03-17 | |||
| WO2018199142A1 | 2018-11-01 |
Foreign References:
| JP2019531062A | 2019-10-31 |
Other References:
JAROONWITCHAWAN THIRANUT; MUANGCHAN PATTAMON; NOISA PARINYA: "Inhibition of FGF signaling accelerates neural crest cell differentiation of human pluripotent stem cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM NL, vol. 481, no. 1, 2 November 2016 (2016-11-02), Amsterdam NL , pages 176 - 181, XP029811490, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2016.10.147
GRIGOR'EVA ELENA V.; MALANKHANOVA TUYANA B.; SURUMBAYEVA AIZHAN; PAVLOVA SOPHIA V.; MININA JULIA M.; KIZILOVA ELENA A.; SULDINA LY: "Generation of GABAergic striatal neurons by a novel iPSC differentiation protocol enabling scalability and cryopreservation of progenitor cells", CYTOTECHNOLOGY, SPRINGER NETHERLANDS, DORDRECHT, vol. 72, no. 5, 9 June 2020 (2020-06-09), Dordrecht, pages 649 - 663, XP037264972, ISSN: 0920-9069, DOI: 10.1007/s10616-020-00406-7
GRIGOR'EVA ELENA V.; MALANKHANOVA TUYANA B.; SURUMBAYEVA AIZHAN; PAVLOVA SOPHIA V.; MININA JULIA M.; KIZILOVA ELENA A.; SULDINA LY: "Generation of GABAergic striatal neurons by a novel iPSC differentiation protocol enabling scalability and cryopreservation of progenitor cells", CYTOTECHNOLOGY, SPRINGER NETHERLANDS, DORDRECHT, vol. 72, no. 5, 9 June 2020 (2020-06-09), Dordrecht, pages 649 - 663, XP037264972, ISSN: 0920-9069, DOI: 10.1007/s10616-020-00406-7
Attorney, Agent or Firm:
UCHIYAMA Tsutomu et al. (JP)
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