Title:
NEW CARBON GLYCOSIDE GLYCOSYLTRANSFERASE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/147575
Kind Code:
A1
Abstract:
Provided is a group of new uridine diphosphate (UDP)-glycosyltransferases, which are carbon glycoside glycosyltransferases, wherein the glycosyltransferases can specifically and efficiently catalyze the carbon glycoside glucosylation of a dihydrochalcone(s) compound or a 2-hydroxyflavanone(s) compound, thereby producing a carbon glycoside dihydrochalcone(s) compound or a carbon glycoside-2-hydroxyflavanone(s) compound; and a flavonoid carbon glycoside(s) compound is formed from a carbon glycoside-2-hydroxyflavanone(s) compound by means of a further dehydration reaction. Further provided is the use of said new UDP glycosyltransferases in artificially constructed recombinant expression systems to produce a carbon glycoside dihydrochalcone(s) compound or a flavonoid carbon glycoside(s) compound by means of fermentation engineering.
Inventors:
WANG YONG (CN)
SUN YUWEI (CN)
CHEN ZHUO (CN)
SUN YUWEI (CN)
CHEN ZHUO (CN)
Application Number:
PCT/CN2020/136886
Publication Date:
July 29, 2021
Filing Date:
December 16, 2020
Export Citation:
Assignee:
CAS CENTER FOR EXCELLENCE IN MOLECULAR PLANT SCIENCES (CN)
International Classes:
C07H15/203; C12N9/10; C12N15/09; C12P19/00
Foreign References:
| CN102292441A | 2011-12-21 | |||
| JP2015204752A | 2015-11-19 | |||
| CN101775050A | 2010-07-14 |
Other References:
GUTMANN ALEXANDER, BUNGARUANG LINDA, WEBER HANSJOERG, LEYPOLD MARIO, BREINBAUER ROLF, NIDETZKY BERND: "Towards the synthesis of glycosylated dihydrochalcone natural products using glycosyltransferase-catalysed cascade reactions", GREEN CHEMISTRY, vol. 16, no. 9, 1 January 2014 (2014-01-01), GB, pages 4417 - 4425, XP055832000, ISSN: 1463-9262, DOI: 10.1039/C4GC00960F
DATABASE PROTEIN 29 September 2019 (2019-09-29), ANONYMOUS: "C-glycosyltransferase [Phyllostachys edulis]", XP055832003, retrieved from GENBANK Database accession no. QES86201
DATABASE PROTEIN 13 June 2017 (2017-06-13), ANONYMOUS: "UDP-glucose:2-hydroxyflavanone C-glucosyltransferase [Sorghum bicolor]", XP055832005, retrieved from GENBANK Database accession no. XP_002438250
ITO TAKAMITSU, FUJIMOTO SHUNSUKE, SUITO FUMIAKI, SHIMOSAKA MAKOTO, TAGUCHI GORO: "C -Glycosyltransferases catalyzing the formation of di- C -glucosyl flavonoids in citrus plants", THE PLANT JOURNAL, vol. 91, no. 2, 1 July 2017 (2017-07-01), GB, pages 187 - 198, XP055807368, ISSN: 0960-7412, DOI: 10.1111/tpj.13555
NOBUHIRO SASAKI, NISHIZAKI YUZO, YAMADA ERI, TATSUZAWA FUMI, NAKATSUKA TAKASHI, TAKAHASHI HIDEYUKI, NISHIHARA MASAHIRO: "Identification of the glucosyltransferase that mediates direct flavone C -glucosylation in Gentiana triflora", FEBS LETTERS, ELSEVIER, vol. 589, no. 1, 2 January 2015 (2015-01-02), NL, pages 182 - 187, XP055590062, ISSN: 0014-5793, DOI: 10.1016/j.febslet.2014.11.045
J. SAMBROOK: "Molecular Cloning: A Laboratory Manual", 2002, SCIENCE PRESS
"GenBank", Database accession no. XP _015642954.1
See also references of EP 4095145A4
DATABASE PROTEIN 29 September 2019 (2019-09-29), ANONYMOUS: "C-glycosyltransferase [Phyllostachys edulis]", XP055832003, retrieved from GENBANK Database accession no. QES86201
DATABASE PROTEIN 13 June 2017 (2017-06-13), ANONYMOUS: "UDP-glucose:2-hydroxyflavanone C-glucosyltransferase [Sorghum bicolor]", XP055832005, retrieved from GENBANK Database accession no. XP_002438250
ITO TAKAMITSU, FUJIMOTO SHUNSUKE, SUITO FUMIAKI, SHIMOSAKA MAKOTO, TAGUCHI GORO: "C -Glycosyltransferases catalyzing the formation of di- C -glucosyl flavonoids in citrus plants", THE PLANT JOURNAL, vol. 91, no. 2, 1 July 2017 (2017-07-01), GB, pages 187 - 198, XP055807368, ISSN: 0960-7412, DOI: 10.1111/tpj.13555
NOBUHIRO SASAKI, NISHIZAKI YUZO, YAMADA ERI, TATSUZAWA FUMI, NAKATSUKA TAKASHI, TAKAHASHI HIDEYUKI, NISHIHARA MASAHIRO: "Identification of the glucosyltransferase that mediates direct flavone C -glucosylation in Gentiana triflora", FEBS LETTERS, ELSEVIER, vol. 589, no. 1, 2 January 2015 (2015-01-02), NL, pages 182 - 187, XP055590062, ISSN: 0014-5793, DOI: 10.1016/j.febslet.2014.11.045
J. SAMBROOK: "Molecular Cloning: A Laboratory Manual", 2002, SCIENCE PRESS
"GenBank", Database accession no. XP _015642954.1
See also references of EP 4095145A4
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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