Title:
MUTEIN, METHOD FOR PRODUCING SAID MUTEIN, GENE ENCODING SAID MUTEIN, RECOMBINANT VECTOR AND PLANT BEARING SAID GENE, METHOD FOR CONTROLLING AMOUNT OF MEVALONIC ACID PRODUCED AND AMOUNT OF ISOPRENOID PRODUCED, METHOD FOR CONTROLLING 3-HYDROXY-3-METHYLGLUTARYL-COA REDUCTASE ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2015/178493
Kind Code:
A1
Abstract:
Provided is a mutein having mutations of specific amino acid residues of HMGR, which is a rate-limiting enzyme of isoprene monomer biosynthesis in the polyisoprenoid biosynthetic pathway. The present invention relates to a mutein in which at least one amino acid residue selected from the group consisting of positions 91, 225, 257, 287, 339, 411, 470, 509, and 574 and amino acid residues of positions corresponding to these in Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-CoA reductase shown by SEQ ID NO: 1 have been deleted or substituted by another amino acid residue in 3-hydroxy-3-methylglutaryl-CoA reductase.
Inventors:
SUZUKI KEIKO (JP)
JEKSON ROBERTLEE (JP)
MURANAKA TOSHIYA (JP)
TAKAGI KEIJI (JP)
INOUE YUKINO (JP)
FUSHIHARA KAZUHISA (JP)
JEKSON ROBERTLEE (JP)
MURANAKA TOSHIYA (JP)
TAKAGI KEIJI (JP)
INOUE YUKINO (JP)
FUSHIHARA KAZUHISA (JP)
Application Number:
PCT/JP2015/064821
Publication Date:
November 26, 2015
Filing Date:
May 22, 2015
Export Citation:
Assignee:
UNIV OSAKA (JP)
SUMITOMO RUBBER IND (JP)
SUMITOMO RUBBER IND (JP)
International Classes:
C12N15/00; A01H5/00; C12N9/04; C12N15/09; C12P5/02; C12P7/40
Domestic Patent References:
| WO2001031043A1 | 2001-05-03 |
Other References:
DATABASE GenBank [O] 18 April 2005 (2005-04-18), CHYE M. L. ET AL.: "hydroxymethylglutaryl-CoA reductase [Hevea brasiliens is", XP055238415, retrieved from ncbi Database accession no. CAA38469.1
DATABASE GenBank [O] 27 April 1993 (1993-04-27), CHYE M. L. ET AL.: "3-hydroxy-3-methylglutaryl- coenzyme A reductase [Hevea brasiliens is", XP055238417, retrieved from ncbi Database accession no. AAA33360.1
DATABASE GenBank 13 September 2008 (2008-09-13), SANDO T. ET AL.: "hydroxymethylglutaryl-CoA reductase [Hevea brasiliens is", XP055238418, retrieved from ncbi Database accession no. BAF98281.1
DATABASE GenBank 13 September 2008 (2008-09-13), SANDO T. ET AL.: "hydroxymethylglutaryl-CoA reductase [Hevea brasiliens is", XP055238420, retrieved from ncbi Database accession no. BAF98280.1
CHING Y. P. ET AL.: "Analysis of the specificity of the AMP-activated protein kinase by site- directed mutagenesis of bacterially expressed 3-hydroxy 3-methylglutaryl-CoA reductase, using a single primer variant of the unique-site- elimination method", EUR. J. BIOCHEM., vol. 237, no. 3, 1996, pages 800 - 8, XP002604746
OMKUMAR R. V. ET AL.: "Modulation of Syrian hamster 3-hydroxy-3-methylglutaryl-CoA reductase activity by phosphorylation", J. BIOL. CHEM., vol. 269, no. 9, 1994, pages 6810 - 6814, XP002159997, ISSN: 0021-9258
See also references of EP 3147359A4
DATABASE GenBank [O] 27 April 1993 (1993-04-27), CHYE M. L. ET AL.: "3-hydroxy-3-methylglutaryl- coenzyme A reductase [Hevea brasiliens is", XP055238417, retrieved from ncbi Database accession no. AAA33360.1
DATABASE GenBank 13 September 2008 (2008-09-13), SANDO T. ET AL.: "hydroxymethylglutaryl-CoA reductase [Hevea brasiliens is", XP055238418, retrieved from ncbi Database accession no. BAF98281.1
DATABASE GenBank 13 September 2008 (2008-09-13), SANDO T. ET AL.: "hydroxymethylglutaryl-CoA reductase [Hevea brasiliens is", XP055238420, retrieved from ncbi Database accession no. BAF98280.1
CHING Y. P. ET AL.: "Analysis of the specificity of the AMP-activated protein kinase by site- directed mutagenesis of bacterially expressed 3-hydroxy 3-methylglutaryl-CoA reductase, using a single primer variant of the unique-site- elimination method", EUR. J. BIOCHEM., vol. 237, no. 3, 1996, pages 800 - 8, XP002604746
OMKUMAR R. V. ET AL.: "Modulation of Syrian hamster 3-hydroxy-3-methylglutaryl-CoA reductase activity by phosphorylation", J. BIOL. CHEM., vol. 269, no. 9, 1994, pages 6810 - 6814, XP002159997, ISSN: 0021-9258
See also references of EP 3147359A4
Attorney, Agent or Firm:
YASUTOMI & Associates (JP)
Patent business corporation Yasutomi international patent firm (JP)
Patent business corporation Yasutomi international patent firm (JP)
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