Title:
MULTIPLEX TARGET GENE EXPRESSION INHIBITION SYSTEM BASED ON SYNTHESIS REGULATOR SRNA AND METHOD OF PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2020/004936
Kind Code:
A1
Abstract:
The present invention relates to a multiplex expression inhibition system based on a synthesis regulator sRNA and a method of producing same, and more specifically, to a combination of vectors comprising a variety of antibiotic-resistance genes, an origins of replication, and a synthesis regulator sRNA; a method of producing same; an sRNA library consisting of said combination of vectors; and a method of screening for a bacterial strain producing an useful substance by using same. The multiplex target gene expression inhibition system according to the present invention is a gene expression regulation system based on a synthesis regulator sRNA that, unlike existing gene knock-out methods, can effectively inhibit the expression of a specific gene without altering the sequence of a target gene; comprises various vectors and thus can be used compatibly with other plasmids usable in prokaryotes; and is designed to enable multiplex introduction, and thus is advantageous in a fast and efficient production of recombinant microorganisms. Furthermore, the present invention provides a technology that enables simple and fast transferring a genome-level synthesis regulation sRNA library to a new platform, and said technology is useful in selection of recombinant microorganisms producing useful substances in high capacity. Accordingly, the present invention is useful in the production of recombinant bacterial strains capable of dramatically increasing the production efficiency of a variety of medically useful metabolites.
Inventors:
LEE SANG YUP (KR)
YOO SEUNG MIN (KR)
YANG DONGSOO (KR)
YOO SEUNG MIN (KR)
YANG DONGSOO (KR)
Application Number:
PCT/KR2019/007729
Publication Date:
January 02, 2020
Filing Date:
June 26, 2019
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
International Classes:
C12N15/113; C12N15/65; C12N15/70; C12P17/10
Domestic Patent References:
| WO2014190627A1 | 2014-12-04 |
Foreign References:
| KR101750855B1 | 2017-06-27 | |||
| KR101575587B1 | 2015-12-09 | |||
| KR101690780B1 | 2016-12-29 |
Other References:
CHO, C. ET AL.: "Efficient gene knockdown in Clostridium acetobutylicum by synthetic small regulatory RNAs", BIOTECHNOLOGY AND BIOENGINEERING, vol. 114, no. 2, February 2017 (2017-02-01), pages 374 - 383, XP055668368
NA, D. ET AL.: "Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs", NATURE BIOTECHNOLOGY, vol. 31, no. 2, February 2013 (2013-02-01), pages 170 - 174, XP055142165, DOI: 10.1038/nbt.2461
NOH, M. ET AL.: "Gene expression knockdown by modulating synthetic small RNA expression in Escherichia coli Cell Systems", CELL SYSTEMS, vol. 5, 25 October 2017 (2017-10-25), pages 418 - 426, XP055668375
LEE, SANG YEOB: "Universal Synthetic Regulatory sRNA Technology for Development of Microbe for Chemicals Mass Production", KAIST CORE TECH TRANSFER DAY, vol. 317, 10 September 2018 (2018-09-10), Seoul, Republic of Korea, pages 1 - 13
NA, D. ET AL.: "Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs", NATURE BIOTECHNOLOGY, vol. 31, no. 2, February 2013 (2013-02-01), pages 170 - 174, XP055142165, DOI: 10.1038/nbt.2461
NOH, M. ET AL.: "Gene expression knockdown by modulating synthetic small RNA expression in Escherichia coli Cell Systems", CELL SYSTEMS, vol. 5, 25 October 2017 (2017-10-25), pages 418 - 426, XP055668375
LEE, SANG YEOB: "Universal Synthetic Regulatory sRNA Technology for Development of Microbe for Chemicals Mass Production", KAIST CORE TECH TRANSFER DAY, vol. 317, 10 September 2018 (2018-09-10), Seoul, Republic of Korea, pages 1 - 13
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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