Title:
MODIFIED RECOMBINANT ADENO-ASSOCIATED VIRUS (AAV)-BINDING PROTEIN AND METHOD OF ANALYSIS OF AAV BASED ON INFECTION ABILITY
Document Type and Number:
WIPO Patent Application WO/2023/140197
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing an improved adeno-associated virus (AAV)-binding protein having an enhanced binding ability to an AAV and a method for analyzing an AAV contained in a sample on the basis of difference in infection ability. This problem is solved by substituting amino acid residues at specific positions in the regions corresponding to extracellular region domain 1 (PKD1) and domain 2 (PKD2) of KIAA0319L (UniProt No. Q8IZA0) with other specific amino acid residues. This problem is solved by a method of analysis of an AAV that comprises: a step for adsorbing the AAV contained in a sample onto an adsorbent which comprises an insoluble carrier and a polypeptide containing at least an amino acid sequence corresponding to extracellular region domain 1 or domain 2 of KIAA0319L immobilized on the carrier; and a step for eluting the AAV adsorbed on the adsorbent with an eluent.
Inventors:
KURIHARA KENTO (JP)
WATANABE KAZUYA (JP)
OMURA KEITA (JP)
YOSHIDA KOUHEI (JP)
MAKINO YURIKO (JP)
TANAKA TORU (JP)
WATANABE KAZUYA (JP)
OMURA KEITA (JP)
YOSHIDA KOUHEI (JP)
MAKINO YURIKO (JP)
TANAKA TORU (JP)
Application Number:
PCT/JP2023/000872
Publication Date:
July 27, 2023
Filing Date:
January 13, 2023
Export Citation:
Assignee:
TOSOH CORP (JP)
International Classes:
C07K17/00; B01J20/24; C07K14/705; C07K17/02; C12M1/34; C12N1/21; C12N7/02; C12N15/12; C12N15/63; C12P21/00; C12Q1/04; C12Q1/70
Foreign References:
| US20180327752A1 | 2018-11-15 |
Other References:
S. PILLAY, N. L. MEYER, A. S. PUSCHNIK, O. DAVULCU, J. DIEP, Y. ISHIKAWA, L. T. JAE, J. E. WOSEN, C. M. NAGAMINE, M. S. CHAPMAN, J: "An essential receptor for adeno-associated virus infection", NATURE, vol. 530, no. 7588, 1 January 2016 (2016-01-01), London, pages 108 - 112, XP055384253, ISSN: 0028-0836, DOI: 10.1038/nature16465
ZHANG RAN; CAO LIN; CUI MENGTIAN; SUN ZIXIAN; HU MINGXU; ZHANG ROUXUAN; STUART WILLIAM; ZHAO XIAOCHU; YANG ZIRUI; LI XUEMING; SUN : "Adeno-associated virus 2 bound to its cellular receptor AAVR", NATURE MICROBIOLOGY, vol. 4, no. 4, 11 February 2019 (2019-02-11), London , pages 675 - 682, XP036739072, DOI: 10.1038/s41564-018-0356-7
SUMMERFORD CANDACE, SAMULSKI R. JUDE: "AAVR: A Multi-Serotype Receptor for AAV", MOLECULAR THERAPY, vol. 24, no. 4, 1 April 2016 (2016-04-01), US , pages 663 - 666, XP055830059, ISSN: 1525-0016, DOI: 10.1038/mt.2016.49
ZHANG RAN; CAO LIN; CUI MENGTIAN; SUN ZIXIAN; HU MINGXU; ZHANG ROUXUAN; STUART WILLIAM; ZHAO XIAOCHU; YANG ZIRUI; LI XUEMING; SUN : "Adeno-associated virus 2 bound to its cellular receptor AAVR", NATURE MICROBIOLOGY, vol. 4, no. 4, 11 February 2019 (2019-02-11), London , pages 675 - 682, XP036739072, DOI: 10.1038/s41564-018-0356-7
SUMMERFORD CANDACE, SAMULSKI R. JUDE: "AAVR: A Multi-Serotype Receptor for AAV", MOLECULAR THERAPY, vol. 24, no. 4, 1 April 2016 (2016-04-01), US , pages 663 - 666, XP055830059, ISSN: 1525-0016, DOI: 10.1038/mt.2016.49
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
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