Title:
MODIFIED LAMININ AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2012/137970
Kind Code:
A1
Abstract:
Provided is a modified laminin characterized in that a cell growth regulatory molecule is bound to at least one site of the N-terminus of an α-chain, the C-terminus of an α-chain, the N-terminus of a β-chain, and the N-terminus of a γ-chain of a laminin or a laminin fragment forming a hetero-trimer. Also provided are a culturing method for culturing cells in the presence of the modified laminin, a method for establishing iPS cells in the presence of the modified laminin, and a culture base material coated with the modified laminin. By culturing human stem cells in a xeno-free environment using the modified laminin of the invention, human stem cells which are highly safe and can be applied to regenerative medicine can be provided.
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Inventors:
SEKIGUCHI KIYOTOSHI (JP)
TANIGUCHI YUKIMASA (JP)
NAKAGAWA MASATO (JP)
TANIGUCHI YUKIMASA (JP)
NAKAGAWA MASATO (JP)
Application Number:
PCT/JP2012/059720
Publication Date:
October 11, 2012
Filing Date:
April 09, 2012
Export Citation:
Assignee:
UNIV OSAKA (JP)
UNIV KYOTO (JP)
SEKIGUCHI KIYOTOSHI (JP)
TANIGUCHI YUKIMASA (JP)
NAKAGAWA MASATO (JP)
UNIV KYOTO (JP)
SEKIGUCHI KIYOTOSHI (JP)
TANIGUCHI YUKIMASA (JP)
NAKAGAWA MASATO (JP)
International Classes:
C07K14/47; C07K14/78; C07K19/00; C12N1/00; C12N5/0735; C12N5/10; C12N15/09
Domestic Patent References:
| WO2008084401A2 | 2008-07-17 | |||
| WO2011043405A1 | 2011-04-14 | |||
| WO2007069666A1 | 2007-06-21 | |||
| WO2008118820A2 | 2008-10-02 | |||
| WO2010098419A1 | 2010-09-02 | |||
| WO2011102531A1 | 2011-08-25 | |||
| WO2011016588A1 | 2011-02-10 |
Foreign References:
| US201161521153P | 2011-08-08 |
Other References:
SHOICHI SASAKI ET AL.: "Shinkei Soshiki Kochiku no Tameno Kinosei Matrix Tanpakushitsu no Sekkei", 90TH ANNUAL MEETING OF THE CHEMICAL SOCIETY OF JAPAN IN SPRING KOEN YOKOSHU, 2010, pages 605, XP008169686
MIYAZAKI,T. ET AL.: "Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells", BIOCHEM. BIOPHYS.RES.COMMUN., vol. 375, no. 1, 2008, pages 27 - 32, XP024520467
RODIN,S. ET AL.: "Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511", NAT.BIOTECHNOL., vol. 28, no. 6, 2010, pages 611 - 615, XP009135150
WELSH,E.R. ET AL.: "Engineering the extracellular matrix: a novel approach to polymeric biomaterials. I. Control of the physical properties of artificial protein matrices designed to support adhesion of vascular endothelial cells", BIOMACROMOLECULES., vol. 1, no. 1, 2000, pages 23 - 30, XP002988982
MCMAHON, A. P.; BRADLEY, A., CELL, vol. 62, 1990, pages 1073 - 1085
MIYAZAKI T; FUTAKI S; HASEGAWA K; KAWASAKI M; SANZEN N; HAYASHI M, KAWASE E; SEKIGUCHI K; NAKATSUJI N; SUEMORI H.: "Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 375, 2008, pages 27 - 35
EDGAR D.; TIMPL R.; THOENEN H.: "The heparin-binding domain of laminin is responsible for its effects on neurite outgrowth and neuronal survival", EMBO J., vol. 3, 1984, pages 1463 - 1468, XP055222218
GOODMAN SL.; DEUTZMANN R.; VON DER MARK K.: "Two distinct cell-binding domains in laminin can independently promote nonneuronal cell adhesion and spreading", J. CELL BIOL., vol. 105, 1987, pages 589 - 598, XP055304702, DOI: doi:10.1083/jcb.105.1.589
HIROYUKI IDO; KENJI HARADA; SUGIKO FUTAKI; YOSHITAKA HAYASHI; RYOKO NISHIUCHI; YUKO NATSUKA; SHAOLIANG LI; YOSHINAO WADA; ARIANA C: "Molecular dissection of the a-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, 2004, pages 10946 - 10954
HIROYUKI IDO; AYA NAKAMURA; REIKO KOBAYASHI; SHUNSUKE ITO; SHAOLIANG LI; SUGIKO FUTAKI; KIYOTOSHI SEKIGUCHI: "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin y chains in integrin binding by laminins", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 282, 2007, pages 11144 - 11154, XP008153460, DOI: doi:10.1074/jbc.M609402200
FUSAO KIMIZUKA; YOICHI OHDATE; YASUTOSHI KAWASE; TOMOKO SHIMOJYO; YUKI TAGUCHI; KIMIKAZU HASHINO; SHOICHI GOTO; HIDETAKA HASHI; IK: "Role of type III homology repeats in cell adhesive function within the cell-binding domain of fibronectin", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 266, 1999, pages 3045 - 3051
RI-ICHIROH MANABE; NAOKO OH-E; TOSHINAGA MAEDA; TOMOHIKO FUKUDA; KIYOTOSHI SEKIGUCHI: "Modulation of cell adhesive activity of fibronectin by the alternatively spliced EDA segment", THE JOURNAL OF CELL BIOLOGY, vol. 139, 1997, pages 295 - 307, XP002193809, DOI: doi:10.1083/jcb.139.1.295
YUYA SATO; TOSHIHIKO UEMURA; KEISUKE MORIMITSU; RYOKO SATO-NISHIUCHI; RI-ICHIROH MANABE; JUNICHI TAKAGI; MASASHI YAMADA; KIYOTOSHI: "Molecular basis of the recognition of nephronectin by integrin ?8?1", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 284, 2009, pages 14524 - 14536, XP055063092, DOI: doi:10.1074/jbc.M900200200
UWE WINZEN; GREGORY J. COLE; WILLI HALFTER: "Agrin is a chimeric proteoglycan with the attachment sites for heparan sulfate/chondroitin sulfate located in two multiple serine-glycine clusters", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, 2008, pages 30106 - 30114, XP055336415, DOI: doi:10.1074/jbc.M212676200
LIU, Y. ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 346, 2006, pages 131 - 139
VALLIER, L. ET AL., J. CELL SCI., vol. 118, 2005, pages 4495 - 4509
LI, Y. ET AL., BIOTECHNOL. BIOENG., vol. 91, 2005, pages 688 - 698
YAO, S. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 103, 2006, pages 6907 - 6912
LU, J. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 103, 2006, pages 5688 - 5693
TAKAHASHI, K.; YAMANAKA, S., CELL, vol. 126, 2006, pages 663 - 676
OKITA, K. ET AL., NATURE, vol. 448, 2007, pages 313 - 317
WERNIG, M. ET AL., NATURE, vol. 448, 2007, pages 318 - 324
MAHERALI, N. ET AL., CELL STEM CELL, vol. 1, 2007, pages 55 - 70
NAKAGAWA, M. ET AL., NAT. BIOTETHNOL., vol. 26, 2008, pages 101 - 106
TAKAHASHI, K. ET AL., CELL, vol. 131, 2007, pages 861 - 872
YU, J. ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 1920
MAEKAWA, M. ET AL., NATURE, vol. 474, 2011, pages 225 - 229
NATURE BIOTECHNOLOGY, vol. 26, 2008, pages 101 - 106
CELL, vol. 126, 2006, pages 663 - 676
CELL, vol. 131, 2007, pages 861 - 872
NAT. CELL BIOL., vol. 11, 2009, pages 197 - 203
NATURE, vol. 451, 2008, pages 141 - 146
SCIENCE, vol. 318, 2007, pages 1917 - 1920
STEM CELLS, vol. 26, 2008, pages 1998 - 2005
CELL RESEARCH, 2008, pages 600 - 603
NATURE, vol. 454, 2008, pages 646 - 650
CELL STEM CELL, vol. 2, 2008, pages 525 - 528
PROC. NATL. ACAD. SCI. USA., vol. 107, 2010, pages 14152 - 14157
CELL, vol. 136, 2009, pages 411 - 419
NATURE, vol. 08436, 2009
SCIENCE, vol. 324, 2009, pages 797 - 801
NATURE, vol. 474, 2011, pages 225 - 229
KANO, F. ET AL., METHODS IN MOLECULAR BIOLOGY, vol. 322, 2006, pages 357 - 365
KONDO, E. ET AL., MOL. CANCER THER., vol. 3, no. 12, 2004, pages 1623 - 1630
"Integrin-binding Sites within the Globular Domain of Human Laminin-10", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, 2004, pages 10946 - 10954
HIROYUKI IDO; KENJI HARADA; YOSHIKO YAGI; KIYOTOSHI SEKIGUCHI: "Probing the integrin-binding site within the globular domain of laminin-511 with the function-blocking monoclonal antibody 4C7", MATRIX BIOLOGY, vol. 25, no. 2, 2006, pages 112 - 117
HIROKI AKAMATSU; KEIKO ICHIHARA-TANAKA; KEIICHI OZONO; WATARU KAMIIKE; HIKARU MATSUDA; KIYOTOSHI SEKIGUCHI: "Suppression of Transformed Phenotypes of Human Fibrosarcoma Cells by Overexpression of Recombinant Fibronectin", CANCER RESEARCH, vol. 56, 1996, pages 4541 - 4546
MASATO NAKAGAWA; NANAKO TAKIZAWA; MEGUMI NARITA; TOMOKO ICHISAKA; SHINYA YAMANAKA: "Promotion of direct reprogramming by transformation-deficient Myc", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 107, no. 32, 2010, pages 14152 - 14157, XP008151220, DOI: doi:10.1073/pnas.1009374107
KAZUTOSHI TAKAHASHI; KOJI TANABE; MARI OHNUKI; MEGUMI NARITA; TOMOKO ICHISAKA; KIICHIRO TOMODA; SHINYA YAMANAKA: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors", CELL, vol. 131, no. 5, 2007, pages 861 - 872, XP008155962, DOI: doi:10.1016/j.cell.2007.11.019
KIYOTOSHI SEKIGUCHI; SEN-ITIROH HAKOMORI: "Domain structure of human plasma fibronectin. Differences and similarities between human and hamster fibronectins", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 258, 1983, pages 3967 - 3973
JUNICHI TAKAGI; HAROLD P. ERICKSON; TIMOTHY A. SPRINGER: "C-terminal opening mimics inside-out activation of integrin ?5?1", NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 8, no. 5, 2001, pages 412 - 416
SHAOLIANG LI; CHISEI SHIMONO; NAOKO NORIOKA; ITSUKO NAKANO; TETSUO OKUBO; YOSHIKO YAGI; MARIA HAYASHI; YUYA SATO; HITOMI FUJISAKI;: "Activin A Binds to Perlecan through Its Pro-region That Has Heparin/Heparan Sulfate Binding Activity", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 285, no. 47, 2010, pages 36645 - 36655, XP055266811, DOI: doi:10.1074/jbc.M110.177865
OKITA ET AL.: "A more efficient method to generate integration-free human iPS cells", NATURE METHODS, vol. 8, no. 5, 2011, pages 409, XP055176852, DOI: doi:10.1038/nmeth.1591
See also references of EP 2695894A4
MIYAZAKI,T. ET AL.: "Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells", BIOCHEM. BIOPHYS.RES.COMMUN., vol. 375, no. 1, 2008, pages 27 - 32, XP024520467
RODIN,S. ET AL.: "Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511", NAT.BIOTECHNOL., vol. 28, no. 6, 2010, pages 611 - 615, XP009135150
WELSH,E.R. ET AL.: "Engineering the extracellular matrix: a novel approach to polymeric biomaterials. I. Control of the physical properties of artificial protein matrices designed to support adhesion of vascular endothelial cells", BIOMACROMOLECULES., vol. 1, no. 1, 2000, pages 23 - 30, XP002988982
MCMAHON, A. P.; BRADLEY, A., CELL, vol. 62, 1990, pages 1073 - 1085
MIYAZAKI T; FUTAKI S; HASEGAWA K; KAWASAKI M; SANZEN N; HAYASHI M, KAWASE E; SEKIGUCHI K; NAKATSUJI N; SUEMORI H.: "Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 375, 2008, pages 27 - 35
EDGAR D.; TIMPL R.; THOENEN H.: "The heparin-binding domain of laminin is responsible for its effects on neurite outgrowth and neuronal survival", EMBO J., vol. 3, 1984, pages 1463 - 1468, XP055222218
GOODMAN SL.; DEUTZMANN R.; VON DER MARK K.: "Two distinct cell-binding domains in laminin can independently promote nonneuronal cell adhesion and spreading", J. CELL BIOL., vol. 105, 1987, pages 589 - 598, XP055304702, DOI: doi:10.1083/jcb.105.1.589
HIROYUKI IDO; KENJI HARADA; SUGIKO FUTAKI; YOSHITAKA HAYASHI; RYOKO NISHIUCHI; YUKO NATSUKA; SHAOLIANG LI; YOSHINAO WADA; ARIANA C: "Molecular dissection of the a-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, 2004, pages 10946 - 10954
HIROYUKI IDO; AYA NAKAMURA; REIKO KOBAYASHI; SHUNSUKE ITO; SHAOLIANG LI; SUGIKO FUTAKI; KIYOTOSHI SEKIGUCHI: "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin y chains in integrin binding by laminins", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 282, 2007, pages 11144 - 11154, XP008153460, DOI: doi:10.1074/jbc.M609402200
FUSAO KIMIZUKA; YOICHI OHDATE; YASUTOSHI KAWASE; TOMOKO SHIMOJYO; YUKI TAGUCHI; KIMIKAZU HASHINO; SHOICHI GOTO; HIDETAKA HASHI; IK: "Role of type III homology repeats in cell adhesive function within the cell-binding domain of fibronectin", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 266, 1999, pages 3045 - 3051
RI-ICHIROH MANABE; NAOKO OH-E; TOSHINAGA MAEDA; TOMOHIKO FUKUDA; KIYOTOSHI SEKIGUCHI: "Modulation of cell adhesive activity of fibronectin by the alternatively spliced EDA segment", THE JOURNAL OF CELL BIOLOGY, vol. 139, 1997, pages 295 - 307, XP002193809, DOI: doi:10.1083/jcb.139.1.295
YUYA SATO; TOSHIHIKO UEMURA; KEISUKE MORIMITSU; RYOKO SATO-NISHIUCHI; RI-ICHIROH MANABE; JUNICHI TAKAGI; MASASHI YAMADA; KIYOTOSHI: "Molecular basis of the recognition of nephronectin by integrin ?8?1", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 284, 2009, pages 14524 - 14536, XP055063092, DOI: doi:10.1074/jbc.M900200200
UWE WINZEN; GREGORY J. COLE; WILLI HALFTER: "Agrin is a chimeric proteoglycan with the attachment sites for heparan sulfate/chondroitin sulfate located in two multiple serine-glycine clusters", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, 2008, pages 30106 - 30114, XP055336415, DOI: doi:10.1074/jbc.M212676200
LIU, Y. ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 346, 2006, pages 131 - 139
VALLIER, L. ET AL., J. CELL SCI., vol. 118, 2005, pages 4495 - 4509
LI, Y. ET AL., BIOTECHNOL. BIOENG., vol. 91, 2005, pages 688 - 698
YAO, S. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 103, 2006, pages 6907 - 6912
LU, J. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 103, 2006, pages 5688 - 5693
TAKAHASHI, K.; YAMANAKA, S., CELL, vol. 126, 2006, pages 663 - 676
OKITA, K. ET AL., NATURE, vol. 448, 2007, pages 313 - 317
WERNIG, M. ET AL., NATURE, vol. 448, 2007, pages 318 - 324
MAHERALI, N. ET AL., CELL STEM CELL, vol. 1, 2007, pages 55 - 70
NAKAGAWA, M. ET AL., NAT. BIOTETHNOL., vol. 26, 2008, pages 101 - 106
TAKAHASHI, K. ET AL., CELL, vol. 131, 2007, pages 861 - 872
YU, J. ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 1920
MAEKAWA, M. ET AL., NATURE, vol. 474, 2011, pages 225 - 229
NATURE BIOTECHNOLOGY, vol. 26, 2008, pages 101 - 106
CELL, vol. 126, 2006, pages 663 - 676
CELL, vol. 131, 2007, pages 861 - 872
NAT. CELL BIOL., vol. 11, 2009, pages 197 - 203
NATURE, vol. 451, 2008, pages 141 - 146
SCIENCE, vol. 318, 2007, pages 1917 - 1920
STEM CELLS, vol. 26, 2008, pages 1998 - 2005
CELL RESEARCH, 2008, pages 600 - 603
NATURE, vol. 454, 2008, pages 646 - 650
CELL STEM CELL, vol. 2, 2008, pages 525 - 528
PROC. NATL. ACAD. SCI. USA., vol. 107, 2010, pages 14152 - 14157
CELL, vol. 136, 2009, pages 411 - 419
NATURE, vol. 08436, 2009
SCIENCE, vol. 324, 2009, pages 797 - 801
NATURE, vol. 474, 2011, pages 225 - 229
KANO, F. ET AL., METHODS IN MOLECULAR BIOLOGY, vol. 322, 2006, pages 357 - 365
KONDO, E. ET AL., MOL. CANCER THER., vol. 3, no. 12, 2004, pages 1623 - 1630
"Integrin-binding Sites within the Globular Domain of Human Laminin-10", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, 2004, pages 10946 - 10954
HIROYUKI IDO; KENJI HARADA; YOSHIKO YAGI; KIYOTOSHI SEKIGUCHI: "Probing the integrin-binding site within the globular domain of laminin-511 with the function-blocking monoclonal antibody 4C7", MATRIX BIOLOGY, vol. 25, no. 2, 2006, pages 112 - 117
HIROKI AKAMATSU; KEIKO ICHIHARA-TANAKA; KEIICHI OZONO; WATARU KAMIIKE; HIKARU MATSUDA; KIYOTOSHI SEKIGUCHI: "Suppression of Transformed Phenotypes of Human Fibrosarcoma Cells by Overexpression of Recombinant Fibronectin", CANCER RESEARCH, vol. 56, 1996, pages 4541 - 4546
MASATO NAKAGAWA; NANAKO TAKIZAWA; MEGUMI NARITA; TOMOKO ICHISAKA; SHINYA YAMANAKA: "Promotion of direct reprogramming by transformation-deficient Myc", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 107, no. 32, 2010, pages 14152 - 14157, XP008151220, DOI: doi:10.1073/pnas.1009374107
KAZUTOSHI TAKAHASHI; KOJI TANABE; MARI OHNUKI; MEGUMI NARITA; TOMOKO ICHISAKA; KIICHIRO TOMODA; SHINYA YAMANAKA: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors", CELL, vol. 131, no. 5, 2007, pages 861 - 872, XP008155962, DOI: doi:10.1016/j.cell.2007.11.019
KIYOTOSHI SEKIGUCHI; SEN-ITIROH HAKOMORI: "Domain structure of human plasma fibronectin. Differences and similarities between human and hamster fibronectins", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 258, 1983, pages 3967 - 3973
JUNICHI TAKAGI; HAROLD P. ERICKSON; TIMOTHY A. SPRINGER: "C-terminal opening mimics inside-out activation of integrin ?5?1", NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 8, no. 5, 2001, pages 412 - 416
SHAOLIANG LI; CHISEI SHIMONO; NAOKO NORIOKA; ITSUKO NAKANO; TETSUO OKUBO; YOSHIKO YAGI; MARIA HAYASHI; YUYA SATO; HITOMI FUJISAKI;: "Activin A Binds to Perlecan through Its Pro-region That Has Heparin/Heparan Sulfate Binding Activity", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 285, no. 47, 2010, pages 36645 - 36655, XP055266811, DOI: doi:10.1074/jbc.M110.177865
OKITA ET AL.: "A more efficient method to generate integration-free human iPS cells", NATURE METHODS, vol. 8, no. 5, 2011, pages 409, XP055176852, DOI: doi:10.1038/nmeth.1591
See also references of EP 2695894A4
Attorney, Agent or Firm:
IWATANI, Ryo (JP)
Tatsu Iwatani (JP)
Tatsu Iwatani (JP)
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