Title:
MICROBIAL STRAIN, PROTEIN, AND METHOD FOR PRODUCING GALLIC ACID USING MICROBIAL STRAIN OR PROTEIN
Document Type and Number:
WIPO Patent Application WO/2024/075674
Kind Code:
A1
Abstract:
The purpose of the present technology is to provide: a protein that has a protocatechuic acid 5-position oxidizing activity and can efficiently generate gallic acid from protocatechuic acid; and a microbial strain that expresses said protein. In the present invention, the use of a protocatechuic acid 5-position oxidizing enzyme derived from Comamonas microbes or a protein having at least 70% identity with the amino acid sequence of said protocatechuic acid 5-position oxidizing enzyme allows efficient production of gallic acid from protocatechuic acid. Gallic acid can be produced from protocatechuic acid as a result of fermentation using a microbial strain into which a gene that codes for said enzyme has been introduced.
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Inventors:
TAKAYA NAOKI (JP)
NUKUI NORIYUKI (JP)
KATSUKI NOZOMI (JP)
MASUO SHUNSUKE (JP)
NUKUI NORIYUKI (JP)
KATSUKI NOZOMI (JP)
MASUO SHUNSUKE (JP)
Application Number:
PCT/JP2023/035854
Publication Date:
April 11, 2024
Filing Date:
October 02, 2023
Export Citation:
Assignee:
UNIV TSUKUBA (JP)
International Classes:
C12N1/21; C12N1/20; C12N9/04; C12N15/53; C12P7/42
Foreign References:
| JP2021185830A | 2021-12-13 | |||
| JP2021185831A | 2021-12-13 | |||
| JP2009213392A | 2009-09-24 |
Other References:
DATABASE UniProtKB 7 April 2021 (2021-04-07), ANONYMOUS: "SubName: Full=4-hydroxybenzoate 3-monooxygenase {ECO:0000313|EMBL:QBK05525.1}; EC=1.14.13.2 {ECO:0000313|EMBL:QBK05525.1}", XP093154745, Database accession no. A0A4P6UN65
DATABASE UniProtKB 7 April 2021 (2021-04-07), ANONYMOUS: "SubName: Full=4-hydroxybenzoate 3-monooxygenase {ECO:0000313|EMBL:QHE86262.1}", XP093154749, Database accession no. A0A6P1IUY4
DATABASE UniProtKB 2 December 2020 (2020-12-02), ANONYMOUS: "SubName: Full=4-hydroxybenzoate 3-monooxygenase {ECO:0000313|EMBL:QDL37155.1}", XP093154752, Database accession no. A0A515D9R9
KATSUKI, NOZOMI ET AL.: "Identification and characterization of novel proteins belonging to p-hydroxybenzoate hydroxylase family", PROCEEDINGS OF THE ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY 2022, 3B09-09, 5 March 2022 (2022-03-05)
DATABASE UniProtKB 7 April 2021 (2021-04-07), ANONYMOUS: "SubName: Full=4-hydroxybenzoate 3-monooxygenase {ECO:0000313|EMBL:QHE86262.1}", XP093154749, Database accession no. A0A6P1IUY4
DATABASE UniProtKB 2 December 2020 (2020-12-02), ANONYMOUS: "SubName: Full=4-hydroxybenzoate 3-monooxygenase {ECO:0000313|EMBL:QDL37155.1}", XP093154752, Database accession no. A0A515D9R9
KATSUKI, NOZOMI ET AL.: "Identification and characterization of novel proteins belonging to p-hydroxybenzoate hydroxylase family", PROCEEDINGS OF THE ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY 2022, 3B09-09, 5 March 2022 (2022-03-05)
Attorney, Agent or Firm:
WATANABE Kaoru (JP)
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