Title:
METHOD FOR SELECTIVE DIFFERENTIATION FROM PLURIPOTENT STEM CELL TO HINDBRAIN TISSUE
Document Type and Number:
WIPO Patent Application WO/2024/035130
Kind Code:
A1
Abstract:
The present invention relates to a method for selective differentiation from a pluripotent stem cell to hindbrain tissue, the method comprising a step of selective differentiation into hindbrain tissue, in which embryoid bodies formed from pluripotent stem cells are cultured in the presence of a hindbrain tissue induction medium containing endogenous WNT secretion inhibitors and WNT signaling agonists.
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Inventors:
KWAK TAE HWAN (KR)
YANG JI HUN (KR)
YANG JI HUN (KR)
Application Number:
PCT/KR2023/011785
Publication Date:
February 15, 2024
Filing Date:
August 09, 2023
Export Citation:
Assignee:
NEXT＆BIO INC (KR)
International Classes:
C12N5/079
Foreign References:
| JP5141016B2 | 2013-02-13 | |||
| CN113924362A | 2022-01-11 |
Other References:
LU JIANFENG; ZHONG XUEFEI; LIU HUISHENG; HAO LING; HUANG CINDY TZU-LING; SHERAFAT MOHAMMAD AMIN; JONES JEFFREY; AYALA MELVIN; LI L: "Generation of serotonin neurons from human pluripotent stem cells", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 34, no. 1, 1 January 2016 (2016-01-01), New York, pages 89 - 94, XP037104139, ISSN: 1087-0156, DOI: 10.1038/nbt.3435
MOYA NOEL, CUTTS JOSH, GAASTERLAND TERRY, WILLERT KARL, BRAFMAN DAVID A.: "Endogenous WNT Signaling Regulates hPSC-Derived Neural Progenitor Cell Heterogeneity and Specifies Their Regional Identity", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 3, no. 6, 1 December 2014 (2014-12-01), United States , pages 1015 - 1028, XP093138175, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2014.10.004
ZHUOQING FANG, XINYUAN LIU, JING WEN, FAN TANG, YANG ZHOU, NAIHE JING, YING JIN: "SOX21 Ensures Rostral Forebrain Identity by Suppression of WNT8B during Neural Regionalization of Human Embryonic Stem Cells", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 13, no. 6, 1 December 2019 (2019-12-01), United States , pages 1038 - 1052, XP055768796, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2019.10.013
MOYA NOEL, CUTTS JOSH, GAASTERLAND TERRY, WILLERT KARL, BRAFMAN DAVID A.: "Endogenous WNT Signaling Regulates hPSC-Derived Neural Progenitor Cell Heterogeneity and Specifies Their Regional Identity", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 3, no. 6, 1 December 2014 (2014-12-01), United States , pages 1015 - 1028, XP093138175, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2014.10.004
ZHUOQING FANG, XINYUAN LIU, JING WEN, FAN TANG, YANG ZHOU, NAIHE JING, YING JIN: "SOX21 Ensures Rostral Forebrain Identity by Suppression of WNT8B during Neural Regionalization of Human Embryonic Stem Cells", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 13, no. 6, 1 December 2019 (2019-12-01), United States , pages 1038 - 1052, XP055768796, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2019.10.013
Attorney, Agent or Firm:
TNI IP LAW FIRM (KR)
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