Title:
METHOD FOR REGULATING PLANT SOMATIC EMBRYOGENESIS
Document Type and Number:
WIPO Patent Application WO/2023/082486
Kind Code:
A1
Abstract:
A method for regulating plant somatic embryogenesis. Plant immature zygotic embryos are used as explants to induce somatic embryogenesis by controlling the co-expression of shoot apical meristem stem cell regulatory factors, miR394 and WUS. According to the present application, test results show that WUS accelerates somatic embryogenesis with dose and stage effects, and miR394 can not only help WUS regulate shoot apical meristem development, but also improve efficiency of WUS to induce somatic embryogenesis, thereby transforming Ler Arabidopsis, of which the somatic embryogenesis is difficult, into a plant more efficient in somatic embryogenesis.
Inventors:
CHEN JINHUI (CN)
LU LU (CN)
LAUX THOMAS (DE)
SHI JISEN (CN)
LU LU (CN)
LAUX THOMAS (DE)
SHI JISEN (CN)
Application Number:
PCT/CN2022/077040
Publication Date:
May 19, 2023
Filing Date:
February 21, 2022
Export Citation:
Assignee:
UNIV NANJING FORESTRY (CN)
UNIV OF FREIBURG (DE)
UNIV OF FREIBURG (DE)
International Classes:
C12N5/04
Foreign References:
| CN101597328A | 2009-12-09 | |||
| CN111235175A | 2020-06-05 | |||
| CN109929852A | 2019-06-25 | |||
| AU2007201633A1 | 2007-05-03 |
Other References:
陈裕坤等 (CHEN, YUKUN ET AL.): "龙眼体细胞胚胎发生研究进展 (Advances in Somatic Embryogenesis of Dimocarpus longan Lour)", 热带作物学报 (CHINESE JOURNAL OF TROPICAL CROPS), vol. 41, no. 10, 24 August 2020 (2020-08-24), XP009546197, ISSN: 1534-5807
ALMA ARMENTA-MEDINA ET AL.: "Arabidopsis thaliana miRNAs promote embryo pattern formation", DEVELOPMENTAL BIOLOGY, vol. 431, no. 2,, 15 November 2017 (2017-11-15), XP085240881, ISSN: 1095-564X
KNAUER STEFFEN, HOLT ANNA L., RUBIO-SOMOZA IGNACIO, TUCKER ELISE J., HINZE ANNIKA, PISCH MELANIE, JAVELLE MARIE, TIMMERMANS MARJA : "A Protodermal miR394 Signal Defines a Region of Stem Cell Competence in the Arabidopsis Shoot Meristem", DEVELOPMENTAL CELL, CELL PRESS, US, vol. 24, no. 2, 1 January 2013 (2013-01-01), US , pages 125 - 132, XP093065397, ISSN: 1534-5807, DOI: 10.1016/j.devcel.2012.12.009
ALMA ARMENTA-MEDINA ET AL.: "Arabidopsis thaliana miRNAs promote embryo pattern formation", DEVELOPMENTAL BIOLOGY, vol. 431, no. 2,, 15 November 2017 (2017-11-15), XP085240881, ISSN: 1095-564X
KNAUER STEFFEN, HOLT ANNA L., RUBIO-SOMOZA IGNACIO, TUCKER ELISE J., HINZE ANNIKA, PISCH MELANIE, JAVELLE MARIE, TIMMERMANS MARJA : "A Protodermal miR394 Signal Defines a Region of Stem Cell Competence in the Arabidopsis Shoot Meristem", DEVELOPMENTAL CELL, CELL PRESS, US, vol. 24, no. 2, 1 January 2013 (2013-01-01), US , pages 125 - 132, XP093065397, ISSN: 1534-5807, DOI: 10.1016/j.devcel.2012.12.009
Attorney, Agent or Firm:
NANJING SHENYUN INTELLECTUAL PROPERTY OFFICE (GENERAL (CN)
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