METHOD FOR RECOVERING ENVIRONMENTAL DNA OR ENVIRONMENTAL RNA

WIPO Patent Application WO/2024/080275

A1

The present invention addresses the problem of providing a method whereby environmental DNA or environmental RNA can be captured using a carrier having a high holding force, and the captured environmental DNA or environmental RNA can subsequently be efficiently recovered from the carrier.　The method performed for recovering environmental DNA or environmental RNA from an environmental sample includes steps (a) and (b), in order, where (a) is a step for bringing the environmental sample containing the environmental DNA or the environmental RNA into contact with a nucleic acid capturing carrier comprising a flexible porous material to capture the environmental DNA or the environmental RNA or a biological sample containing the environmental DNA or the environmental RNA on the nucleic acid capturing carrier, and (b) is a step for positively or negatively pressurizing the nucleic acid capturing carrier.

AKAMATSU YOSHIHISA (JP)
NAKAO RYOHEI (JP)
INABA MANAMI (JP)
IWAKI FUMIKO (JP)

PCT/JP2023/036721

April 18, 2024

October 10, 2023

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UNIV YAMAGUCHI (JP)
NIPPON KOEI CO LTD (JP)

C12N15/10

WO2010082631A1

2010-07-22

JP2013516186A	2013-05-13
JP2013042670A	2013-03-04
JP2001521818A	2001-11-13

INABA, MANAMI ET AL.: "Development of a passive sampling method for the virus concentration from the wastewater using dried sea sponges", LECTURES OF 56TH ANNUAL CONFERENCE OF JAPAN SOCIETY ON WALER ENVIRONMENT, 10 March 2022 (2022-03-10), pages 94
YOSHIHISA AKAMATSU: "Fundamental study for the development of a passive sampling method on the environmental DNA surveys", ABSTRACTS OF THE 4TH ANNUAL MEETING OF EDNA SOCIETY, 1 November 2021 (2021-11-01), XP093158690

FUJIMOTO Shohei (JP)

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