Title:
METHOD AND RECOMBINANT STRAIN FOR ENHANCING METHYL MERCURY DEMETHYLATION AND/OR DIVALENT MERCURY REDUCTION ABILITIES OF FUNGUS, AND USE
Document Type and Number:
WIPO Patent Application WO/2023/092655
Kind Code:
A1
Abstract:
A method and recombinant strain for enhancing the methyl mercury demethylation and/or divalent mercury reduction abilities of a fungus, and the use, relating to the technical field of recombinant strains. Using the method, a recombinant fungus that overexpresses the methyl mercury demethylase gene Mmd or bivalent mercury reductase Mir from Metarhizium robertsii alone and a recombinant engineering fungus that simultaneously expresses the Mmd and Mir genes can be respectively obtained. The methyl mercury demethylation and/or divalent mercury reduction abilities of the recombinant fungus and the recombinant engineering fungus can be significantly enhanced compared with wild-type fungi.
Inventors:
FANG WEIGUO (CN)
WU CONGCONG (CN)
BAO YUTING (CN)
WU CONGCONG (CN)
BAO YUTING (CN)
Application Number:
PCT/CN2021/136433
Publication Date:
June 01, 2023
Filing Date:
December 08, 2021
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
C12N1/15; B09C1/10; C12N1/19; C12N15/80; C12N15/81; C12R1/645; C12R1/865
Foreign References:
| CN103525849A | 2014-01-22 |
Other References:
DATABASE Protein 26 February 2015 (2015-02-26), ANONYMOUS : "alkylmercury lyase [Metarhizium robertsii ARSEF 23] ", XP093068803, retrieved from Genbank Database accession no. XP_007825874
DATABASE Protein 26 February 2015 (2015-02-26), ANONYMOUS : "Pyruvate/2-oxoglutarate dehydrogenase [Metarhizium robertsii ARSEF 23]", XP093068807, retrieved from Genbank Database accession no. XP_007824121
RUIZ OSCAR N., HUSSEIN HUSSEIN S., TERRY NORMAN, DANIELL HENRY: "Phytoremediation of Organomercurial Compounds via Chloroplast Genetic Engineering", PLANT PHYSIOLOGY, vol. 132, no. 3, 31 July 2003 (2003-07-31), Rockville, Md, USA , pages 1344 - 1352, XP093068809, ISSN: 0032-0889, DOI: 10.1104/pp.103.020958
ZHANG MEIHUA;SUN LU;ZHU TONG;WANG XINHONG;JIANG JIHONG;ZHAO SHENGKAI: "Isolation of a Mercury-resistant Fungal Strain and Its Aquatic Bio-sorption Behavior of Hg（Ⅱ)", ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 38, no. 12Q, 31 December 2015 (2015-12-31), pages 1 - 6, XP093068814, ISSN: 1003-6504, DOI: 10.3969/j.issn.1003-6504.2015.12Q.001
CHRISTAKIS CHRISTOS A., BARKAY TAMAR, BOYD ERIC S.: "Expanded Diversity and Phylogeny of mer Genes Broadens Mercury Resistance Paradigms and Reveals an Origin for MerA Among Thermophilic Archaea", FRONTIERS IN MICROBIOLOGY, vol. 12, 1 January 2021 (2021-01-01), pages 1 - 20, XP093068820, DOI: 10.3389/fmicb.2021.682605
LU JIA-MI,LIU YAN-SHENG,ZHOU XIAO-YANG: "Heavy Metal Transporters and Their Applications in Phytoremediation", CHINESE JOURNAL OF ECO-AGRICULTURE, vol. 15, no. 1, 30 January 2007 (2007-01-30), pages 195 - 200, XP093068824
DATABASE Protein 26 February 2015 (2015-02-26), ANONYMOUS : "Pyruvate/2-oxoglutarate dehydrogenase [Metarhizium robertsii ARSEF 23]", XP093068807, retrieved from Genbank Database accession no. XP_007824121
RUIZ OSCAR N., HUSSEIN HUSSEIN S., TERRY NORMAN, DANIELL HENRY: "Phytoremediation of Organomercurial Compounds via Chloroplast Genetic Engineering", PLANT PHYSIOLOGY, vol. 132, no. 3, 31 July 2003 (2003-07-31), Rockville, Md, USA , pages 1344 - 1352, XP093068809, ISSN: 0032-0889, DOI: 10.1104/pp.103.020958
ZHANG MEIHUA;SUN LU;ZHU TONG;WANG XINHONG;JIANG JIHONG;ZHAO SHENGKAI: "Isolation of a Mercury-resistant Fungal Strain and Its Aquatic Bio-sorption Behavior of Hg（Ⅱ)", ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 38, no. 12Q, 31 December 2015 (2015-12-31), pages 1 - 6, XP093068814, ISSN: 1003-6504, DOI: 10.3969/j.issn.1003-6504.2015.12Q.001
CHRISTAKIS CHRISTOS A., BARKAY TAMAR, BOYD ERIC S.: "Expanded Diversity and Phylogeny of mer Genes Broadens Mercury Resistance Paradigms and Reveals an Origin for MerA Among Thermophilic Archaea", FRONTIERS IN MICROBIOLOGY, vol. 12, 1 January 2021 (2021-01-01), pages 1 - 20, XP093068820, DOI: 10.3389/fmicb.2021.682605
LU JIA-MI,LIU YAN-SHENG,ZHOU XIAO-YANG: "Heavy Metal Transporters and Their Applications in Phytoremediation", CHINESE JOURNAL OF ECO-AGRICULTURE, vol. 15, no. 1, 30 January 2007 (2007-01-30), pages 195 - 200, XP093068824
Attorney, Agent or Firm:
BEIJING FINELAND IP FIRM (CN)
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