Title:
METHOD FOR PRODUCING OLIGONUCLEOTIDE
Document Type and Number:
WIPO Patent Application WO/2024/089953
Kind Code:
A1
Abstract:
The present invention provides a method for producing oligonucleotide by a solid-phase synthesis technique, wherein said method for producing oligonucleotide comprises a step for reacting, in the presence of a thiol, an acid with an oligonucleotide in which the hydroxyl group at the 5' end is protected by a protection group that is removable under acidic conditions, in order to remove the protection group on the hydroxyl group at the 5' end. The present invention also provides oligonucleotide in which the content ratio of N-1-mer in the oligonucleotide is not greater than a certain amount.
Inventors:
TANAKA YUKI (JP)
Application Number:
PCT/JP2023/026108
Publication Date:
May 02, 2024
Filing Date:
July 14, 2023
Export Citation:
Assignee:
SUMITOMO CHEMICAL CO (JP)
International Classes:
C07H21/02; C07H1/00; C12N15/11
Domestic Patent References:
| WO2019208571A1 | 2019-10-31 | |||
| WO2022172994A1 | 2022-08-18 |
Foreign References:
| JP2005520547A | 2005-07-14 |
Other References:
MITSUAKI SEKIGUCHI: "Consideration for Impurities Contained in Oligonucleotide Therapeutics", PHARMACEUTICAL AND MEDICAL DEVICE REGULATORY SCIENCE, vol. 51, no. 1, 1 January 2020 (2020-01-01), pages 11 - 21, XP093165672
N. M. EL ZAHAR; N. MAGDY; A. M. EL‐KOSASY; MICHAEL G. BARTLETT: "Chromatographic approaches for the characterization and quality control of therapeutic oligonucleotide impurities", BIOMEDICAL CHROMATOGRAPHY, JOHN WILEY & SONS LTD., GB, vol. 32, no. 1, 4 October 2017 (2017-10-04), GB , pages e4088, XP071550514, ISSN: 0269-3879, DOI: 10.1002/bmc.4088
N. M. EL ZAHAR; N. MAGDY; A. M. EL‐KOSASY; MICHAEL G. BARTLETT: "Chromatographic approaches for the characterization and quality control of therapeutic oligonucleotide impurities", BIOMEDICAL CHROMATOGRAPHY, JOHN WILEY & SONS LTD., GB, vol. 32, no. 1, 4 October 2017 (2017-10-04), GB , pages e4088, XP071550514, ISSN: 0269-3879, DOI: 10.1002/bmc.4088
Attorney, Agent or Firm:
YAMAO, Norihito et al. (JP)
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