Title:
METHOD FOR PRODUCING COMPOUND-K BY USING PHOTOSYNTHETIC BACTERIUM
Document Type and Number:
WIPO Patent Application WO/2023/140608
Kind Code:
A1
Abstract:
The present invention relates to the preparation of a recombinant strain to which a gene coding for a recombinant photosynthetic enzyme into which squalene epoxidase and cytochrome P450 reductase are translationally fused, a gene coding for a recombinant enzyme in which dammarenediol II synthase, protopanaxadiol synthase, and cytochrome 450 reductase are translationally fused, and a gene coding for UDP-glycosyltransferase are introduced, and a method for production of ginsenoside compound-K by using same, with squalene serving as a starting substrate. The method of the present invention can secure a yield of compound-K of 0.74 g/L or more per day, and can achieve maximum product yield efficiently and quickly in a short time, compared to conventional methods.
Inventors:
LEE JEONG KUG (KR)
KIM HYEON JUN (KR)
TONG XIAOMENG (KR)
KIM HYEON JUN (KR)
TONG XIAOMENG (KR)
Application Number:
PCT/KR2023/000852
Publication Date:
July 27, 2023
Filing Date:
January 18, 2023
Export Citation:
Assignee:
UNIV SOGANG RES & BUSINESS DEVELOPMENT FOUND (KR)
International Classes:
C12P33/20; C12N1/20
Foreign References:
| CN105087739A | 2015-11-25 |
Other References:
XING YAN, YUN FAN, WEI WEI, PINGPING WANG, QUNFANG LIU, YONGJUN WEI, LEI ZHANG, GUOPING ZHAO, JIANMIN YUE, ZHIHUA ZHOU: "Production of bioactive ginsenoside compound K in metabolically engineered yeast", CELL RESEARCH, SPRINGER SINGAPORE, SINGAPORE, vol. 24, no. 6, 1 June 2014 (2014-06-01), Singapore , pages 770 - 773, XP055428161, ISSN: 1001-0602, DOI: 10.1038/cr.2014.28
LI DASHUAI; ZHANG QIANG; ZHOU ZHIJIANG; ZHAO FANGLONG; LU WENYU: "Heterologous biosynthesis of triterpenoid dammarenediol-II in engineeredEscherichia coli", BIOTECHNOLOGY LETTERS, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, vol. 38, no. 4, 6 January 2016 (2016-01-06), Dordrecht , pages 603 - 609, XP035648239, ISSN: 0141-5492, DOI: 10.1007/s10529-015-2032-9
DATABASE NUCLEOTIDE ANONYMOUS : "Arabidopsis thaliana P450 reductase 1 (ATR1), mRNA", XP093079432, retrieved from NCBI
DATABASE NUCLEOTIDE ANONYMOUS : "Panax ginseng UGT1 mRNA, complete cds", XP093079434, retrieved from NCBI
LI DASHUAI; ZHANG QIANG; ZHOU ZHIJIANG; ZHAO FANGLONG; LU WENYU: "Heterologous biosynthesis of triterpenoid dammarenediol-II in engineeredEscherichia coli", BIOTECHNOLOGY LETTERS, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, vol. 38, no. 4, 6 January 2016 (2016-01-06), Dordrecht , pages 603 - 609, XP035648239, ISSN: 0141-5492, DOI: 10.1007/s10529-015-2032-9
DATABASE NUCLEOTIDE ANONYMOUS : "Arabidopsis thaliana P450 reductase 1 (ATR1), mRNA", XP093079432, retrieved from NCBI
DATABASE NUCLEOTIDE ANONYMOUS : "Panax ginseng UGT1 mRNA, complete cds", XP093079434, retrieved from NCBI
Attorney, Agent or Firm:
DANA PATENT LAW FIRM (KR)
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