Title:
METHOD FOR PRODUCING 24-METHYLENECHOLESTEROL AS MAIN INGREDIENT OF ROYAL JELLY BY USING NANNOCHLOROPSIS OCULATA IN SEAWATER
Document Type and Number:
WIPO Patent Application WO/2023/060761
Kind Code:
A1
Abstract:
The present invention relates to the technical field of genetic engineering, in particular to an engineering strain of nannochloropsis oculata, and a preparation method therefor and the use thereof. The engineering strain is nannochloropsis oculata with the DWARF1 (DWF1) gene knockout. In the present invention, the strain with the DWF1 gene knockout is constructed by using CRISPR CAS9 technology on the premise of not introducing any exogenous molecular label, so that the engineering strain with significantly reduced cholesterol content and significantly increased 24-methylenecholesterol content is obtained, wherein the inheritable character of the engineering strain is stable. 4-Methylenecholesterol is a main ingredient of royal jelly, and has antihypertensive, anti-arrhythmia and vasospasmolytic activities, etc. This engineering strain, if produced industrially, helps to fill the gap in the field of microalgae with high 24-methylenecholesterol content, achieves the development of a healthy food product with high 24-methylenecholesterol content, high eicosapentaenoic acid content and low cholesterol content by using marine nannochloropsis oculata, and provides a theoretical basis for the industrial production of artificial royal jelly by using seawater and carbon dioxide.
Inventors:
LU YANDU (CN)
DENG XIANGZI (CN)
ZHOU WENXU (CN)
GAN QINHUA (CN)
DENG XIANGZI (CN)
ZHOU WENXU (CN)
GAN QINHUA (CN)
Application Number:
PCT/CN2021/139526
Publication Date:
April 20, 2023
Filing Date:
December 20, 2021
Export Citation:
Assignee:
UNIV HAINAN (CN)
International Classes:
C12N1/13; C12N15/53; C12P33/00
Foreign References:
| CN114058515A | 2022-02-18 | |||
| CN104946535A | 2015-09-30 | |||
| JP2020031572A | 2020-03-05 |
Other References:
LU ET AL.: "Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica", "BIOTECHNOLOGY FOR BIOFUELS", vol. 7, 30 May 2014 (2014-05-30), XP021188563, ISSN: 1754-6834, DOI: 10.1186/1754-6834-7-81
WANG FENG-RU, WANG ZHI-YONG: "The Research of Brassinosteroids Signal Transduction", HUABEI NONGXUE BAO , ACTA AGRICULTURAE BOREALI-SINICA, HEBEI SHENG NONGLIN KEXUEYUAN, CN, vol. 23, no. S2, 28 October 2008 (2008-10-28), CN , pages 29 - 39, XP093057581, ISSN: 1000-7091
CECCHIN MICHELA, BERTEOTTI SILVIA, PALTRINIERI STEFANIA, VIGLIANTE IVANO, IADAROLA BARBARA, GIOVANNONE BARBARA, MAFFEI MASSIMO E.,: "Improved lipid productivity in Nannochloropsis gaditana in nitrogen-replete conditions by selection of pale green mutants", BIOTECHNOLOGY FOR BIOFUELS, vol. 13, no. 1, 1 December 2020 (2020-12-01), XP093057061, DOI: 10.1186/s13068-020-01718-8
WANG FENG-RU, WANG ZHI-YONG: "The Research of Brassinosteroids Signal Transduction", HUABEI NONGXUE BAO , ACTA AGRICULTURAE BOREALI-SINICA, HEBEI SHENG NONGLIN KEXUEYUAN, CN, vol. 23, no. S2, 28 October 2008 (2008-10-28), CN , pages 29 - 39, XP093057581, ISSN: 1000-7091
CECCHIN MICHELA, BERTEOTTI SILVIA, PALTRINIERI STEFANIA, VIGLIANTE IVANO, IADAROLA BARBARA, GIOVANNONE BARBARA, MAFFEI MASSIMO E.,: "Improved lipid productivity in Nannochloropsis gaditana in nitrogen-replete conditions by selection of pale green mutants", BIOTECHNOLOGY FOR BIOFUELS, vol. 13, no. 1, 1 December 2020 (2020-12-01), XP093057061, DOI: 10.1186/s13068-020-01718-8
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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