Title:
METHOD FOR PREPARING URSODEOXYCHOLIC ACID VIA CHEMICAL-ENZYMATIC PROCESS
Document Type and Number:
WIPO Patent Application WO/2018/227940
Kind Code:
A1
Abstract:
A method for preparing ursodeoxycholic acid via a chemical-enzymatic process, comprising: adding hyodeoxycholic acid to a first organic solvent, and oxidising under the effect of an oxidising agent to obtain 6-oxo-lithocholic acid; adding the 6-oxo-lithocholic acid and a sulphonyl hydrazide derivative to a second organic solvent, so that the 6-oxo-lithocholic acid and the sulphonyl hydrazide derivative undergo a nucleophilic addition-elimination reaction to obtain a hydrazone compound; in an inert gas environment, using a reducing agent to reduce the hydrazone compound to obtain lithocholic acid; performing a hydroxylation reaction on the lithocholic acid under the catalytic effect of a hydroxylase and a coenzyme to obtain ursodeoxycholic acid. The method uses hyodeoxycholic acid as an initial raw material, and obtains ursodeoxycholic acid via a three-step chemical process and a one-step biological enzymatic process. The overall synthesis process has few steps, simple operations, high yields and low costs, and may be widely applied to industrial scale production.
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Inventors:
FU RONGZHAO (CN)
LIU LIHUI (CN)
JIANG MING (CN)
LIU YUFENG (CN)
LIU LIHUI (CN)
JIANG MING (CN)
LIU YUFENG (CN)
Application Number:
PCT/CN2017/119977
Publication Date:
December 20, 2018
Filing Date:
December 29, 2017
Export Citation:
Assignee:
BONTAC BIO ENG SHENZHEN CO LTD (CN)
JIANGXI BONTAC GREEN BIOCATALYSIS ECOINDUSTRIAL PARK CO LTD (CN)
JIANGXI BONTAC GREEN BIOCATALYSIS ECOINDUSTRIAL PARK CO LTD (CN)
International Classes:
C12P33/06; C07J9/00
Foreign References:
| CN106701882A | 2017-05-24 | |||
| CN106977572A | 2017-07-25 | |||
| CN1520295A | 2004-08-11 | |||
| CN1106611A | 1995-08-09 | |||
| CN106636285A | 2017-05-10 |
Attorney, Agent or Firm:
SCIHEAD IP LAW FIRM (CN)
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