Title:
METHOD FOR PREPARING PYRROLIDONE
Document Type and Number:
WIPO Patent Application WO/2024/050928
Kind Code:
A1
Abstract:
Disclosed in the present invention is a method for preparing pyrrolidone. Provided in the present invention is a method for preparing pyrrolidone from γ-aminobutyric acid under the catalysis of carnitine-CoA ligase CaiC. The amino acid sequence of the carnitine-CoA ligase CaiC is as shown in SEQ ID NO: 1. The ligase has an activity of catalyzing the cyclization of γ-aminobutyric acid to generate pyrrolidone. By means of taking γ-aminobutyric acid as a substrate of the carnitine-CoA ligase provided by the present invention, the yield of pyrrolidone is 3.26 g/L in 24 h, and the molar yield can reach 39.53%, which reduces the production cycle, increases the yield of pyrrolidone, and accelerates an industrialization process for producing pyrrolidone by means of an enzymatic transformation method.
Inventors:
WU JING (CN)
JIANG XULING (CN)
LIU LIMING (CN)
SONG WEI (CN)
ZHOU YIWEN (CN)
CHEN XIULAI (CN)
LIU JIA (CN)
GAO CONG (CN)
JIANG XULING (CN)
LIU LIMING (CN)
SONG WEI (CN)
ZHOU YIWEN (CN)
CHEN XIULAI (CN)
LIU JIA (CN)
GAO CONG (CN)
Application Number:
PCT/CN2022/126215
Publication Date:
March 14, 2024
Filing Date:
October 19, 2022
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12P17/10; C12N1/21; C12N9/00; C12R1/19
Foreign References:
| CN107438667A | 2017-12-05 | |||
| CN103189520A | 2013-07-03 | |||
| CN108191731A | 2018-06-22 |
Other References:
BERNAL V., ARENSE P., BLATZ V., MANDRAND-BERTHELOT M.A., CÁNOVAS M., IBORRA J.L.: "Role of betaine:CoA ligase (CaiC) in the activation of betaines and the transfer of coenzyme A in Escherichia coli", JOURNAL OF APPLIED MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 105, no. 1, 1 July 2008 (2008-07-01), GB , pages 42 - 50, XP093146979, ISSN: 1364-5072, DOI: 10.1111/j.1365-2672.2008.03740.x
DATABASE Protein 7 March 2022 (2022-03-07), ANONYMOUS : "MULTISPECIES: crotonobetaine/carnitine-CoA ligase [Escherichia] ", XP093146980, retrieved from NCBI Database accession no. WP_001350478.1
DATABASE Protein 7 March 2022 (2022-03-07), ANONYMOUS : "MULTISPECIES: crotonobetaine/carnitine-CoA ligase [Escherichia] ", XP093146980, retrieved from NCBI Database accession no. WP_001350478.1
Attorney, Agent or Firm:
CENTRAL SOUTH WELL INTELLECTUAL PROPERTY OFFICE (CN)
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