Title:
METHOD FOR PREPARING MESENCHYMAL STEM CELL, MESENCHYMAL STEM CELL, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/066156
Kind Code:
A1
Abstract:
The present invention provides a method for preparing a mesenchymal stem cell (MSC), a mesenchymal stem cell, and use thereof. According to the method, a trophoblast stem cell (TSC) is obtained by means of inducing EPSC differentiation, and TSC differentiation is further induced to obtain a mature and stable MSC, such that the MSC yield is greatly improved. The MSC obtained by means of the differentiation in the present invention proliferates rapidly with a doubling time of about 1.5 days, and after the continuous passage is performed 16 times or more, the expression level of a surface marker of the MSC is still kept normal. The EPSC-derived MSC can be prepared in a large amount by means of the passage. In addition, the MSC prepared from the present invention has the capability of secreting FGF4 at a high level, and is conducive to being developed into a cell therapeutic agent for treating type 2 diabetes.
Inventors:
GONG SHIXIN (CN)
GU YUCHUN (CN)
LI NAN (CN)
PENG QINQING (CN)
WU LIDA (CN)
GU YUCHUN (CN)
LI NAN (CN)
PENG QINQING (CN)
WU LIDA (CN)
Application Number:
PCT/CN2023/074764
Publication Date:
April 04, 2024
Filing Date:
February 07, 2023
Export Citation:
Assignee:
ALLIFE REGENERATIVE MEDICINE TECH BEIJING CO LTD (CN)
International Classes:
C12N5/0775; A61K35/28; A61P3/10
Foreign References:
| CN115305234A | 2022-11-08 | |||
| CN114762725A | 2022-07-19 | |||
| CN108884436A | 2018-11-23 | |||
| CN113966393A | 2022-01-21 | |||
| US20180201904A1 | 2018-07-19 |
Other References:
XUEFEI GAO: "Establishment of porcine and human expanded potential stem cells", NATURE CELL BIOLOGY, NATURE PUBLISHING GROUP UK, LONDON, vol. 21, no. 6, 1 June 2019 (2019-06-01), London, pages 687 - 699, XP093153619, ISSN: 1465-7392, DOI: 10.1038/s41556-019-0333-2
XIAOFANG WANG: "Immune modulatory mesenchymal stem cells derived from human embryonic stem cells through a trophoblast-like stage", STEM CELLS, WILEY, vol. 34, no. 2, 1 February 2016 (2016-02-01), pages 380 - 391, XP093153620, ISSN: 1066-5099, DOI: 10.1002/stem.2242
CHEN ZHEN-ZHEN: "Progress on derivation of human embryonic stem cell", CHINESE BULLETIN OF LIFE SCIENCES, vol. 30, no. 8, 25 August 2018 (2018-08-25), pages 906 - 910, XP093153624, DOI: 10.13376/j.cbls/2018108
XIAOFANG WANG: "Immune modulatory mesenchymal stem cells derived from human embryonic stem cells through a trophoblast-like stage", STEM CELLS, WILEY, vol. 34, no. 2, 1 February 2016 (2016-02-01), pages 380 - 391, XP093153620, ISSN: 1066-5099, DOI: 10.1002/stem.2242
CHEN ZHEN-ZHEN: "Progress on derivation of human embryonic stem cell", CHINESE BULLETIN OF LIFE SCIENCES, vol. 30, no. 8, 25 August 2018 (2018-08-25), pages 906 - 910, XP093153624, DOI: 10.13376/j.cbls/2018108
Attorney, Agent or Firm:
BEIJING ZHI CHENG HAN FANG IP AGENCY CO., LTD. (CN)
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