Title:
A METHOD OF MONITORING THE HEALTH OF A SUBJECT
Document Type and Number:
WIPO Patent Application WO/2023/014305
Kind Code:
A3
Abstract:
This disclosure relates to a method of assessing/monitoring the health of a subject, the method comprising subjecting a pancreas-associated polynucleotide to one or more amplification step in the presence of a mixture comprising a control nuclei acid, a surfactant and an oligonucleotide primer and/or probe capable of hybridizing with the target nucleic acid, wherein the oligonucleotide primer and/or probe comprises a cleavage site and a cleavable 3' end. In one embodiment, the pancreas-associated polynucleotide is cell-free RNA and the method comprises annealing the target nucleic acid and performing a reverse transcription prior to the one or more amplification step. Also disclosed are primer and/or primer sets as disclosed herein and kits for use thereof.
Inventors:
KOH LIAN CHYE WINSTON (SG)
KHOO CHIN MENG (SG)
TAI E SHYONG (SG)
KHOO CHIN MENG (SG)
TAI E SHYONG (SG)
Application Number:
PCT/SG2022/050563
Publication Date:
May 04, 2023
Filing Date:
August 05, 2022
Export Citation:
Assignee:
AGENCY SCIENCE TECH & RES (SG)
NAT UNIV SINGAPORE (SG)
NAT UNIV SINGAPORE (SG)
International Classes:
C12Q1/6883; C12Q1/6806; C12Q1/6848; C12Q1/6851; C12Q1/6853; C12Q1/686
Domestic Patent References:
| WO2017156310A1 | 2017-09-14 | |||
| WO2020240031A1 | 2020-12-03 |
Foreign References:
| US20180105881A1 | 2018-04-19 | |||
| US20130310269A1 | 2013-11-21 |
Other References:
DUTRA LARA, FRANZ OLE, PUUPPONEN VELI-MIKKO, TIIROLA MARJA: "DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen", BIOTECHNIQUES, vol. 69, no. 6, 1 December 2020 (2020-12-01), US , pages 450 - 454, XP093043586, ISSN: 0736-6205, DOI: 10.2144/btn-2020-0076
GIJAVANEKAR CHARUL; STRYCH ULRICH; FOFANOV YURIY; FOX GEORGE E.; WILLSON RICHARD C.: "Rare target enrichment for ultrasensitive PCR detection using cot–rehybridization and duplex-specific nuclease", ANALYTICAL BIOCHEMISTRY, vol. 421, no. 1, 1 January 1900 (1900-01-01), Amsterdam, NL , pages 81 - 85, XP028884218, ISSN: 0003-2697, DOI: 10.1016/j.ab.2011.11.010
GIJAVANEKAR CHARUL; STRYCH ULRICH; FOFANOV YURIY; FOX GEORGE E.; WILLSON RICHARD C.: "Rare target enrichment for ultrasensitive PCR detection using cot–rehybridization and duplex-specific nuclease", ANALYTICAL BIOCHEMISTRY, vol. 421, no. 1, 1 January 1900 (1900-01-01), Amsterdam, NL , pages 81 - 85, XP028884218, ISSN: 0003-2697, DOI: 10.1016/j.ab.2011.11.010
Attorney, Agent or Firm:
DONALDSON & BURKINSHAW LLP (SG)
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