Title:
METHOD AND KIT FOR IDENTIFYING BACTERIA THAT CAUSE SEPSIS
Document Type and Number:
WIPO Patent Application WO/2022/203008
Kind Code:
A1
Abstract:
Disclosed is a method for identifying bacteria that cause sepsis, wherein the method includes: a step for performing PCR using a sample collected from a subject and a combination of sets of primers each specific to the full length or a partial region of the gyrB gene of Escherichia coli, the nuc gene of Staphylococcus aureus, the atlE gene of Staphylococcus epidermidis, the gyrB gene of Klebsiella pneumoniae, and the rpoB gene of Enterococcus spp.; and a step for detecting any amplification of the full length or the partial region of each of these genes using a combination of probes each specific to the DNA of the full length or the partial region.
Inventors:
TATEDA KAZUHIRO (JP)
MIYATAKE YUYA (JP)
SAKAI KENTAROU (JP)
HIGUCHI MIZUHO (JP)
YAMAUCHI KAZUAKI (JP)
MIYATAKE YUYA (JP)
SAKAI KENTAROU (JP)
HIGUCHI MIZUHO (JP)
YAMAUCHI KAZUAKI (JP)
Application Number:
PCT/JP2022/014051
Publication Date:
September 29, 2022
Filing Date:
March 24, 2022
Export Citation:
Assignee:
DENKA COMPANY LTD (JP)
TOHO UNIV (JP)
TOHO UNIV (JP)
International Classes:
C12Q1/689; C12N15/10; C12N15/11; C12Q1/686; C12R1/19; C12R1/22; C12R1/445; C12R1/45
Domestic Patent References:
| WO2021201091A1 | 2021-10-07 |
Foreign References:
| JP2013048619A | 2013-03-14 | |||
| US20190054227A1 | 2019-02-21 |
Other References:
ABD EL-AZIZ N.K., A.A GHARIB,E.A.A. MOHAMED,A.H. HUSSEIN: "Real-time PCR versus MALDI-TOF MS and culture-based techniques for diagnosis of bloodstream and pyogenic infections in humans and animals", JOURNAL OF APPLIED MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 130, no. 5, 18 October 2020 (2020-10-18), GB , pages 1630 - 1644, XP055970816, ISSN: 1364-5072, DOI: 10.1111/jam.14862
LEHMUSVUORI A., SOIKKELI M., TUUNAINEN E., SEPPÄ T., SPANGAR A., RANTAKOKKO-JALAVA K., VON LODE P., KARHUNEN U., SOUKKA T., WITTFO: "Ready to use dry-reagent PCR assays for the four common bacterial pathogens using switchable lanthanide luminescence probe system", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM,, NL, vol. 118, 1 November 2015 (2015-11-01), NL , pages 64 - 69, XP055970818, ISSN: 0167-7012, DOI: 10.1016/j.mimet.2015.08.022
BARBIER FREBOURG NOËLLE, LEFEBVRE SOPHIE, BAERT STÉPHANIE, LEMELAND JEAN-FRANÇOIS: "PCR-Based Assay for Discrimination between Invasive and Contaminating Staphylococcus epidermidis Strains", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 38, no. 2, 1 February 2000 (2000-02-01), pages 877 - 880, XP055970823, DOI: 10.1128/jcm.38.2.877-880.2000
HUANG MIN; LIN LI; WU YI-XIN; HONHING HO; HE PENG-FEI; LI GUO-ZHI; HE PENG-BO; XIONG GUO-RU; YUAN YUAN; HE YUE-QIU: "Pathogenicity ofKlebsiella pneumonia(KpC4) infecting maize and mice", JOURNAL OF INTEGRATIVE AGRICULTURE, ELSEVIER, AMSTERDAM, NL, vol. 15, no. 7, 1 January 1900 (1900-01-01), AMSTERDAM, NL, pages 1510 - 1520, XP029635440, ISSN: 2095-3119, DOI: 10.1016/S2095-3119(16)61334-5
ARABESTANI MOHAMMAD REZA, HOSSEIN FAZZELI, BAHRAM NASR ESFAHANI: "Identification of the most common pathogenic bacteria in patients with suspected sepsis by multiplex PCR", THE JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, vol. 8, no. 4, 15 April 2014 (2014-04-15), pages 461 - 468, XP055970827, DOI: 10.3855/jidc.3856
LEHMUSVUORI A., SOIKKELI M., TUUNAINEN E., SEPPÄ T., SPANGAR A., RANTAKOKKO-JALAVA K., VON LODE P., KARHUNEN U., SOUKKA T., WITTFO: "Ready to use dry-reagent PCR assays for the four common bacterial pathogens using switchable lanthanide luminescence probe system", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM,, NL, vol. 118, 1 November 2015 (2015-11-01), NL , pages 64 - 69, XP055970818, ISSN: 0167-7012, DOI: 10.1016/j.mimet.2015.08.022
BARBIER FREBOURG NOËLLE, LEFEBVRE SOPHIE, BAERT STÉPHANIE, LEMELAND JEAN-FRANÇOIS: "PCR-Based Assay for Discrimination between Invasive and Contaminating Staphylococcus epidermidis Strains", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 38, no. 2, 1 February 2000 (2000-02-01), pages 877 - 880, XP055970823, DOI: 10.1128/jcm.38.2.877-880.2000
HUANG MIN; LIN LI; WU YI-XIN; HONHING HO; HE PENG-FEI; LI GUO-ZHI; HE PENG-BO; XIONG GUO-RU; YUAN YUAN; HE YUE-QIU: "Pathogenicity ofKlebsiella pneumonia(KpC4) infecting maize and mice", JOURNAL OF INTEGRATIVE AGRICULTURE, ELSEVIER, AMSTERDAM, NL, vol. 15, no. 7, 1 January 1900 (1900-01-01), AMSTERDAM, NL, pages 1510 - 1520, XP029635440, ISSN: 2095-3119, DOI: 10.1016/S2095-3119(16)61334-5
ARABESTANI MOHAMMAD REZA, HOSSEIN FAZZELI, BAHRAM NASR ESFAHANI: "Identification of the most common pathogenic bacteria in patients with suspected sepsis by multiplex PCR", THE JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, vol. 8, no. 4, 15 April 2014 (2014-04-15), pages 461 - 468, XP055970827, DOI: 10.3855/jidc.3856
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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