Title:
METHOD FOR EFFICIENTLY PURIFYING PHOSPHOLIPID FROM COENZYME Q10 FERMENTED POWDER
Document Type and Number:
WIPO Patent Application WO/2024/082327
Kind Code:
A1
Abstract:
A method for efficiently purifying phospholipid from coenzyme Q10 fermented powder, comprising the following steps: 1) mother liquor culture, 2) seed tank culture, 3) fermentation tank culture, 4) fermented liquor centrifugation, 5) high-pressure homogenization, 6) bacteria crushing liquor centrifugation, 7) vacuum freeze drying, 8) subcritical extraction, 9) acetone-based extraction, and 10) rotary evaporation. By adjusting components of a culture medium and optimizing a culture technology, high-density culture of agrobacterium tumefaciens bacteria can be achieved, a large number of bacteria can be obtained, the content of coenzyme Q10 in a fermented liquor can reach 5.1 g/L, and the content of phospholipid in the fermented liquor can reach 13.5 g/L. Moreover, further disclosed is a separation and purification method for efficiently extracting high-purity phospholipid from coenzyme Q10 fermented powder. The present invention can turn waste into wealth, has good economic benefits, simple and mild steps, and low requirements for a device, and is environmentally friendly.
Inventors:
WU XINMING (CN)
JIANG QI (CN)
JIANG QI (CN)
Application Number:
PCT/CN2022/127865
Publication Date:
April 25, 2024
Filing Date:
October 27, 2022
Export Citation:
Assignee:
HUNAN XIANG MIN PHARMACEUTICAL CO LTD (CN)
International Classes:
C12P7/66; C07C46/10; C07F9/10; C12N1/20; C12N1/38; C12P9/00; C12R1/01
Foreign References:
| CN109956856A | 2019-07-02 | |||
| CN111996219A | 2020-11-27 | |||
| CN101705258A | 2010-05-12 | |||
| US20190376153A1 | 2019-12-12 | |||
| GB1591048A | 1981-06-10 | |||
| JP2000245492A | 2000-09-12 | |||
| US4714571A | 1987-12-22 |
Attorney, Agent or Firm:
BEIJING SKYINTEL PATENT AGENCY (CN)
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