Title:
METHOD FOR DETERMINING THE CONTENT OF FREE SUBSTANCE USING ULTRA FILTRATION-EQUILIBRIUM DIALYSIS CONVERSION
Document Type and Number:
WIPO Patent Application WO/2023/179804
Kind Code:
A1
Abstract:
The present invention belongs to the technical field of free substance testing and relates to a method for determining the content of free substances using ultra filtration-equilibrium dialysis conversion. When performing pre-processing for testing a free substance for an existing high-performance liquid chromatography tandem mass spectrometry technique, although processing by an ultra filtration method is efficient and rapid, the temperature during processing is difficult to control, causing a relatively large error in a measured result; when using an equilibrium dialysis method, although the temperature is easy to control, and a measured result is more accurate, said method is very time-consuming, and can not meet a requirement for rapid result-finding analysis of a clinical biological sample. The present invention provides a method for determining the content of a free substance using ultra filtration-equilibrium dialysis conversion; a free substance is separated from a sample by means of an ultra filtration method to obtain first concentration data; the free substance is separated from a sample by using an equilibrium dialysis method to obtain second concentration data; and a linear equation is established by utilizing the first concentration data and the second concentration data. Requirements of high testing accuracy and not being time-consuming can both be met.
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Inventors:
PENG JUN (CA)
JIANG ZHENZUO (CN)
JIANG ZHENZUO (CN)
Application Number:
PCT/CN2023/092700
Publication Date:
September 28, 2023
Filing Date:
May 08, 2023
Export Citation:
Assignee:
HEFEI NOVA MS BIOTECH MEDICAL EQUIPMENT CO LTD (CN)
International Classes:
G01N30/02; G01N30/06; G01N30/08; G01N30/14; G01N30/72
Domestic Patent References:
| WO2016019037A1 | 2016-02-04 |
Foreign References:
| CN114594187A | 2022-06-07 | |||
| US20060223188A1 | 2006-10-05 | |||
| CN113049719A | 2021-06-29 | |||
| CN113390978A | 2021-09-14 | |||
| CN103454360A | 2013-12-18 |
Attorney, Agent or Firm:
WEN PARTNERS, P.C. (CN)
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