Title:
METHOD FOR DETECTING NEURODEGENERATIVE DISEASE USING SHORT-CHAIN RNA
Document Type and Number:
WIPO Patent Application WO/2023/090436
Kind Code:
A1
Abstract:
Disclosed is a method that enables the detection of the presence or absence of a neurodegenerative disease in a subject more simply and effectively than conventional methods. This method comprises: step (a) for preparing an extracellular vesicle fraction from a body fluid sample of a subject; step (b) for counting the extracellular vesicles contained in the extracellular vesicle fraction obtained in step (a) to obtain the number of extracellular vesicles; step (c) for measuring the total amount of short-chain RNA contained in all extracellular vesicles counted in step (b) to obtain the total amount of short-chain RNA per extracellular vesicle; and step (d) for, when the total amount of short-chain RNA per extracellular vesicle obtained in step (c) is greater than the total amount of short-chain RNA per extracellular vesicle obtained from body fluid samples from healthy subjects, then determining that the subject suffers from a neurodegenerative disease.
Inventors:
KASHIMURA CHIORI (JP)
SUDO HIROKO (JP)
SUDO HIROKO (JP)
Application Number:
PCT/JP2022/042927
Publication Date:
May 25, 2023
Filing Date:
November 18, 2022
Export Citation:
Assignee:
TORAY INDUSTRIES (JP)
International Classes:
C12Q1/6837; C12M1/00; C12N15/09; C12Q1/6851; G01N33/53
Other References:
OMORI, CHIORI ET AL.: "P024 Changes of microRNA Expression in Brain Nervous System in Alzheimer's Disease", JOURNAL OF JAPAN SOCIETY FOR DEMENTIA RESEARCH, vol. 35, no. 4, 15 October 2021 (2021-10-15), pages 612, XP009546643
YUAN QIAN, LI XIAO-DONG, ZHANG SI-MIAO, WANG HONG-WEI, WANG YUN-LIANG: "Extracellular vesicles in neurodegenerative diseases: Insights and new perspectives", GENES & DISEASES, ELSEVIER BV, NL, vol. 8, no. 2, 1 March 2021 (2021-03-01), NL , pages 124 - 132, XP093068935, ISSN: 2352-3042, DOI: 10.1016/j.gendis.2019.12.001
JOILIN GREIG, LEIGH P. NIGEL, NEWBURY SARAH F., HAFEZPARAST MAJID: "An Overview of MicroRNAs as Biomarkers of ALS", FRONTIERS IN NEUROLOGY, vol. 10, XP055805938, DOI: 10.3389/fneur.2019.00186
MANNA IDA, DE BENEDITTIS SELENE, QUATTRONE ANDREA, MAISANO DOMENICO, IACCINO ENRICO, QUATTRONE ALDO: "Exosomal miRNAs as Potential Diagnostic Biomarkers in Alzheimer’s Disease", PHARMACEUTICALS, vol. 13, no. 9, pages 243, XP093068937, DOI: 10.3390/ph13090243
YUAN QIAN, LI XIAO-DONG, ZHANG SI-MIAO, WANG HONG-WEI, WANG YUN-LIANG: "Extracellular vesicles in neurodegenerative diseases: Insights and new perspectives", GENES & DISEASES, ELSEVIER BV, NL, vol. 8, no. 2, 1 March 2021 (2021-03-01), NL , pages 124 - 132, XP093068935, ISSN: 2352-3042, DOI: 10.1016/j.gendis.2019.12.001
JOILIN GREIG, LEIGH P. NIGEL, NEWBURY SARAH F., HAFEZPARAST MAJID: "An Overview of MicroRNAs as Biomarkers of ALS", FRONTIERS IN NEUROLOGY, vol. 10, XP055805938, DOI: 10.3389/fneur.2019.00186
MANNA IDA, DE BENEDITTIS SELENE, QUATTRONE ANDREA, MAISANO DOMENICO, IACCINO ENRICO, QUATTRONE ALDO: "Exosomal miRNAs as Potential Diagnostic Biomarkers in Alzheimer’s Disease", PHARMACEUTICALS, vol. 13, no. 9, pages 243, XP093068937, DOI: 10.3390/ph13090243
Attorney, Agent or Firm:
TANIGAWA AND PARTNERS, PATENT FIRM (JP)
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