Title:
METHOD FOR BREEDING IMPROVED VARIETY OF FORAGE SORGHUM-BACILLUS MEGATERIUM SYMBIONT
Document Type and Number:
WIPO Patent Application WO/2024/083061
Kind Code:
A1
Abstract:
The present invention belongs to the cross technique field of plant-microorganism, and specifically relates to a method for breeding an improved variety of a forage sorghum-bacillus megaterium symbiont. The method comprises the following steps: when a stigma of a female parent is exerted for 2-3 d, spraying a bacillus megaterium solution to the stigma, then pollinating same with pollens from anthers of a male parent after the anthers are exerted for 2-5 d, and harvesting mature seeds. Further disclosed in the present invention is a CAPS marker for rapidly identifying bacillus megaterium BM18-2. The CAPS marker mainly comprises a primer pair designed for an SNP site and endonuclease BspT104I, and an upstream primer and a downstream primer of the primer pair are respectively shown as SEQ ID Nos: 1-2. Provided in the present invention is the first method for breeding the improved variety of the forage sorghum containing endophytic bacillus megaterium. Produced seeds can significantly increase the biomass, crude protein, soluble sugar content and roughage grading index (GI) of plants grown in soil with a salt content ≤8‰; and the CAPS marker for identifying bacillus megaterium BM18-2 in the present invention can realize rapid qualitative identification of bacillus megaterium BM18-2.
Inventors:
ZHONG XIAOXIAN (CN)
MAO ZHENGFENG (CN)
XUE WENTAO (CN)
LIU ZHIWEI (CN)
QIAN CHEN (CN)
WU JUANZI (CN)
MAO ZHENGFENG (CN)
XUE WENTAO (CN)
LIU ZHIWEI (CN)
QIAN CHEN (CN)
WU JUANZI (CN)
Application Number:
PCT/CN2023/124650
Publication Date:
April 25, 2024
Filing Date:
October 16, 2023
Export Citation:
Assignee:
JIANGSU ACAD AGRICULTURAL SCI (CN)
International Classes:
A01G7/06; A01B79/02; A01H1/02; C12N1/20; C12Q1/689
Foreign References:
| CN116018954A | 2023-04-28 | |||
| CN103275891A | 2013-09-04 | |||
| US20160113288A1 | 2016-04-28 | |||
| CN108102984A | 2018-06-01 | |||
| CN110358697A | 2019-10-22 | |||
| KR20050118010A | 2005-12-15 |
Other References:
BIRGIT MITTER, NIKOLAUS PFAFFENBICHLER, RICHARD FLAVELL, ST?PHANE COMPANT, LIVIO ANTONIELLI, ALEXANDRA PETRIC, TERESA BERNINGER, M: "A New Approach to Modify Plant Microbiomes and Traits by Introducing Beneficial Bacteria at Flowering into Progeny Seeds", FRONTIERS IN MICROBIOLOGY, vol. 8, XP055386409, DOI: 10.3389/fmicb.2017.00011
Attorney, Agent or Firm:
NANJING TIANHUA PATENT AGENT CO., LTD. (CN)
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