Title:
LIVE BACTERIA QUANTIFYING DEVICE, QUANTIFYING METHOD, AND KIT FOR QUANTIFYING LIVE BACTERIA
Document Type and Number:
WIPO Patent Application WO/2024/004371
Kind Code:
A1
Abstract:
Provided is a live bacteria quantifying method comprising: a deposition step in which bacteria is placed in a measuring vessel containing a redox substance that is permeable to the cell membranes of the live bacteria to be measured, that is oxidized or reduced within the cells, and that is deposited within the cells, and the bacteria and redox substance are mixed for a prescribed period of time; a current peak measurement step in which the measurement solution is electrochemically measured after the deposition step to determine the current peak of the residual redox substance; and a quantitative evaluation step in which the current peak difference is determined between the current peak of the residual redox substance measured in the current peak measurement step and the current peak of the initial redox substance measured in advance, and the live bacteria are quantitatively evaluated from the current peak difference.
Inventors:
SHIIGI HIROSHI (JP)
IKEDA HIKARU (JP)
YAMAMOTO YOJIRO (JP)
TOKONAMI AKIRA (JP)
NISHII SHIGEKI (JP)
IKEDA HIKARU (JP)
YAMAMOTO YOJIRO (JP)
TOKONAMI AKIRA (JP)
NISHII SHIGEKI (JP)
Application Number:
PCT/JP2023/016896
Publication Date:
January 04, 2024
Filing Date:
April 28, 2023
Export Citation:
Assignee:
UNIV OSAKA PUBLIC CORP (JP)
International Classes:
C12N1/20; C12M1/34; C12Q1/06; G01N27/48
Foreign References:
| JP2004337029A | 2004-12-02 |
Other References:
ISHIKI KENGO, NGUYEN DUNG Q., MORISHITA AYA, SHIIGI HIROSHI, NAGAOKA TSUTOMU: "Electrochemical Detection of Viable Bacterial Cells Using a Tetrazolium Salt", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 90, no. 18, 18 September 2018 (2018-09-18), US , pages 10903 - 10909, XP093125040, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.8b02404
Attorney, Agent or Firm:
UNIUS PATENT ATTORNEYS OFFICE (JP)
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