Title:
IMMUNOASSSAY METHOD AND SYSTEM
Document Type and Number:
WIPO Patent Application WO/2024/040802
Kind Code:
A1
Abstract:
An immunoassay method and system, belonging to the field of nano material testing. The immunoassay method comprises: enabling incident light to be reflected for multiple times in a functional magnetic nanoparticle immunoassay reagent under excitation of an alternating current magnetic field; converting emergent light that has undergone multiple reflections into a voltage signal, and extracting a magneto-optical signal corresponding to the immunoassay reagent; mixing the immunoassay reagent with a solution to be tested, so that the incident light is reflected for multiple times in the mixed solution under the excitation of the alternating current magnetic field; converting the emergent light that has undergone multiple reflections into a voltage signal, and extracting a magneto-optical signal corresponding to the mixed solution; and when the difference degree of the magneto-optical signal corresponding to the immunoassay reagent and the magneto-optical signal corresponding to the mixed solution exceeds a preset threshold value, determining that the solution to be tested contains a target molecule to be tested. The method can lower the lower limit of test concentration of molecules to be tested, and improve the precision and sensitivity of immunoassay. The method has convenience of no-wash, and achieves simple operation compared with existing magneto-optical immunoassay methods.
Inventors:
LIU WENZHONG (CN)
ZHU YIWEN (CN)
WANG LIN (CN)
ZHOU YIMING (CN)
CUI XINCHAO (CN)
LI LAN (CN)
ZHU YIWEN (CN)
WANG LIN (CN)
ZHOU YIMING (CN)
CUI XINCHAO (CN)
LI LAN (CN)
Application Number:
PCT/CN2022/138911
Publication Date:
February 29, 2024
Filing Date:
December 14, 2022
Export Citation:
Assignee:
UNIV HUAZHONG SCIENCE TECH (CN)
SHENZHEN HUAZHONG UNIV OF SCIENCE AND TECHNOLOGY RESEARCH INSTITUTE (CN)
SHENZHEN HUAZHONG UNIV OF SCIENCE AND TECHNOLOGY RESEARCH INSTITUTE (CN)
International Classes:
G01N21/21; G01N33/543
Foreign References:
| CN115326716A | 2022-11-11 | |||
| CN105531578A | 2016-04-27 | |||
| CN106596473A | 2017-04-26 | |||
| CN110057785A | 2019-07-26 | |||
| US20200386680A1 | 2020-12-10 | |||
| US4725140A | 1988-02-16 | |||
| US5238811A | 1993-08-24 |
Attorney, Agent or Firm:
HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY PATENT AGENCY CENTER (CN)
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