Title:
HYPERSPECTRAL SEQUENCING METHOD AND SYSTEM AND GENE SEQUENCER
Document Type and Number:
WIPO Patent Application WO/2023/241076
Kind Code:
A1
Abstract:
A hyperspectral sequencing method and system and a gene sequencer. The method comprises: emitting laser light to a sample to be tested, so that a base in the sample generates excitation fluorescence; collecting the excitation fluorescence and performing diffraction and imaging, and obtaining a fluorescence image containing a corresponding fluorescence spectrum after diffraction; determining a target fluorescence spectrum, and identifying, in the fluorescence image, target diffracted light having a light splitting effect in the target fluorescence spectrum; and obtaining a wavelength of a corresponding target base according to the target diffracted light, and determining a base category according to the wavelength. The system comprises a light source module, an optical path module, an imaging module, and a processing module; in the optical path module, a laser (111) emits laser light, the laser light comes to a dichroic mirror (121) through an optical path and then is reflected to reach an objective lens (122), and passes through the objective lens (122) and then a line illumination light spot is presented on a sample to be tested (131), and one-dimensional scanning imaging is performed on said sample (131).
Inventors:
CHEN LONGCHAO (CN)
LIANG QIAN (CN)
ZHANG FEN (CN)
WANG GUFENG (CN)
ZHAO LUYANG (CN)
LIANG QIAN (CN)
ZHANG FEN (CN)
WANG GUFENG (CN)
ZHAO LUYANG (CN)
Application Number:
PCT/CN2023/076185
Publication Date:
December 21, 2023
Filing Date:
February 15, 2023
Export Citation:
Assignee:
SHENZHEN SALUS BIOMED CO LTD (CN)
International Classes:
C12M1/34; G01N21/64; C12Q1/6874; G01N21/01
Foreign References:
| CN115266662A | 2022-11-01 | |||
| US20180024061A1 | 2018-01-25 | |||
| JP2009145320A | 2009-07-02 | |||
| US20100157086A1 | 2010-06-24 | |||
| CN108700460A | 2018-10-23 | |||
| CN112970092A | 2021-06-15 | |||
| DE102018218122A1 | 2020-04-23 |
Attorney, Agent or Firm:
GUANGZHOU PRIME IP LAW OFFICE (CN)
Download PDF: